Transcript: Human NM_022832.4

Homo sapiens ubiquitin specific peptidase 46 (USP46), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
USP46 (64854)
Length:
7932
CDS:
152..1252

Additional Resources:

NCBI RefSeq record:
NM_022832.4
NBCI Gene record:
USP46 (64854)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_022832.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000350480 CGTGGGCATTATATCACTATT pLKO_005 1082 CDS 100% 13.200 18.480 N USP46 n/a
2 TRCN0000022402 GCTAAACACTATTGCGGACAT pLKO.1 535 CDS 100% 4.050 5.670 N USP46 n/a
3 TRCN0000315060 GCTAAACACTATTGCGGACAT pLKO_005 535 CDS 100% 4.050 5.670 N USP46 n/a
4 TRCN0000022401 CGCTTACCAATGAAACTCGAT pLKO.1 675 CDS 100% 2.640 3.696 N USP46 n/a
5 TRCN0000350404 CGCTTACCAATGAAACTCGAT pLKO_005 675 CDS 100% 2.640 3.696 N USP46 n/a
6 TRCN0000315062 CTATGGCCTGACGTCAGATAT pLKO_005 1180 CDS 100% 13.200 10.560 N USP46 n/a
7 TRCN0000315061 TTCAGTCTGACATAGAGTTAA pLKO_005 1435 3UTR 100% 13.200 9.240 N USP46 n/a
8 TRCN0000022400 CCAGAGCAGTTTCCAATCAAT pLKO.1 224 CDS 100% 5.625 3.938 N USP46 n/a
9 TRCN0000022399 GACAGAAGAATTGGGTAGATA pLKO.1 1701 3UTR 100% 5.625 3.938 N USP46 n/a
10 TRCN0000022403 AGCAAACAAGAAGCCCAGAAA pLKO.1 851 CDS 100% 4.950 3.465 N USP46 n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 6284 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 6160 3UTR 100% 2.640 1.320 Y LINC01098 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 6284 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_022832.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08876 pDONR223 100% 99.9% 100% None 1098G>A n/a
2 ccsbBroad304_08876 pLX_304 0% 99.9% 100% V5 1098G>A n/a
3 TRCN0000481093 AAGTTCAGCCCTGAACCCGCATGT pLX_317 43.7% 99.9% 100% V5 1098G>A n/a
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