Transcript: Human NM_024319.4

Homo sapiens chromosome 1 open reading frame 35 (C1orf35), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
C1orf35 (79169)
Length:
1290
CDS:
99..890

Additional Resources:

NCBI RefSeq record:
NM_024319.4
NBCI Gene record:
C1orf35 (79169)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_024319.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000286021 TCAACTGGGAGGACGTGAAGA pLKO_005 145 CDS 100% 4.950 3.960 N C1orf35 n/a
2 TRCN0000277709 TTGGCTACAAGAACGTGAAGA pLKO_005 343 CDS 100% 4.950 3.960 N C1orf35 n/a
3 TRCN0000121942 CCAGATTTATCAGTAGAGGCT pLKO.1 1136 3UTR 100% 0.660 0.528 N C1orf35 n/a
4 TRCN0000277651 CCAGATTTATCAGTAGAGGCT pLKO_005 1136 3UTR 100% 0.660 0.528 N C1orf35 n/a
5 TRCN0000277653 CTGTCTGTGTTCACGCATCAC pLKO_005 531 CDS 100% 4.050 2.835 N C1orf35 n/a
6 TRCN0000160606 CAAGAAAGAGAAGAAGAAGAA pLKO.1 689 CDS 100% 4.950 2.970 N C1orf35 n/a
7 TRCN0000122032 GAAACACAAGAAAGAGAAGAA pLKO.1 683 CDS 100% 4.950 2.970 N C1orf35 n/a
8 TRCN0000159258 GAAAGAGAAGAAGAAGAAAGA pLKO.1 692 CDS 100% 4.950 2.970 N C1orf35 n/a
9 TRCN0000277650 GAAAGAGAAGAAGAAGAAAGA pLKO_005 692 CDS 100% 4.950 2.970 N C1orf35 n/a
10 TRCN0000121643 GAGAAGAAGAAGAAAGACAAA pLKO.1 696 CDS 100% 4.950 2.970 N C1orf35 n/a
11 TRCN0000158715 GAGGAAACACAAGAAAGAGAA pLKO.1 680 CDS 100% 4.950 2.970 N C1orf35 n/a
12 TRCN0000159276 GAAGAAGAAGAAAGACAAAGA pLKO.1 698 CDS 100% 4.950 2.475 Y C1orf35 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024319.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04063 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04063 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000466455 TCTTTCTTTGGGTTTCAATTTTCG pLX_317 34.8% 100% 100% V5 n/a
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