Transcript: Mouse NM_025730.3

Mus musculus leucine-rich repeat kinase 2 (Lrrk2), mRNA.

Source:
NCBI, updated 2017-06-25
Taxon:
Mus musculus (mouse)
Gene:
Lrrk2 (66725)
Length:
8231
CDS:
58..7641

Additional Resources:

NCBI RefSeq record:
NM_025730.3
NBCI Gene record:
Lrrk2 (66725)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025730.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000322256 AGACATCCTCGTCACTATATT pLKO_005 7684 3UTR 100% 15.000 21.000 N Lrrk2 n/a
2 TRCN0000322193 GGCCGAGTTGTGGATCATATT pLKO_005 5389 CDS 100% 13.200 18.480 N Lrrk2 n/a
3 TRCN0000322191 GGCTTACTACTTCACGATATT pLKO_005 6235 CDS 100% 13.200 18.480 N Lrrk2 n/a
4 TRCN0000022656 CGCAGCTTTCAGCGATTCTAA pLKO.1 7095 CDS 100% 5.625 7.875 N Lrrk2 n/a
5 TRCN0000322254 CGCAGCTTTCAGCGATTCTAA pLKO_005 7095 CDS 100% 5.625 7.875 N Lrrk2 n/a
6 TRCN0000022655 GCTTACTACTTCACGATATTT pLKO.1 6236 CDS 100% 15.000 10.500 N Lrrk2 n/a
7 TRCN0000022654 GCCCTGTATATTGCCAAGAAA pLKO.1 7141 CDS 100% 5.625 3.938 N Lrrk2 n/a
8 TRCN0000022658 GCTGCCATCATTGCGAAGATT pLKO.1 6082 CDS 100% 5.625 3.938 N Lrrk2 n/a
9 TRCN0000022657 CCTAAGAACATCATTGTTGAA pLKO.1 6496 CDS 100% 4.950 3.465 N Lrrk2 n/a
10 TRCN0000322192 TCCACTTTGCAGCGCTTTAAA pLKO_005 1954 CDS 100% 15.000 9.000 N Lrrk2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025730.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000465878 ACACTGGGATAATCTTGTACGCTG pLX_317 4.4% 82.7% 86.3% V5 (many diffs) n/a
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