Construct: ORF TRCN0000465878
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006776.1_s317c1
- Derived from:
- BRDN0000395114
- DNA Barcode:
- ACACTGGGATAATCTTGTACGCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRRK2 (120892)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465878
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | NM_198578.4 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_005268629.4 | 99.6% | 99.4% | (many diffs) |
| 3 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_011537877.3 | 98.8% | 98.7% | (many diffs) |
| 4 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_024448833.1 | 84% | 83.9% | (many diffs) |
| 5 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XR_001748574.2 | 66.2% | (many diffs) | |
| 6 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_011537881.3 | 63.6% | 63.5% | (many diffs) |
| 7 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_017018786.2 | 60.2% | 59.6% | (many diffs) |
| 8 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_017018787.1 | 59.1% | 59% | (many diffs) |
| 9 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_017018788.2 | 50.3% | 50% | (many diffs) |
| 10 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_011537882.3 | 50.2% | 49.7% | (many diffs) |
| 11 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_017018789.2 | 50% | 49.7% | (many diffs) |
| 12 | mouse | 66725 | Lrrk2 | leucine-rich repeat kinase 2 | NM_025730.3 | 82.7% | 86.3% | (many diffs) |
| 13 | mouse | 66725 | Lrrk2 | leucine-rich repeat kinase 2 | XM_006521277.3 | 67% | 70.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 7650
- ORF length:
- 7581
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctagtggc aggtgtcagt ggtgcgaaga ggacgaggaa actctgaaga 121 agttgatagt caggctgaac aatgtccagg aaggaaaaca gatagaaacg ctggtccaaa 181 tcctggagga tctgctggtg ttcacgtact ccgagcacgc ctccaagtta tttcaaggca 241 aaaatatcca tgtgcctctg ttgatcgtct tggactccta tatgagagtc gcgagtgtgc 301 agcaggtggg ttggtcactt ctgtgcaaat taatagaagt ctgtccaggt acaatgcaaa 361 gcttaatggg accccaggat gttggaaatg attgggaagt ccttggtgtt caccaattga 421 ttcttaaaat gctaacagtt cataatgcca gtgtaaactt gtcagtgatt ggactgaaga 481 ccttagatct cctcctaact tcaggtaaaa tcaccttgct gatactggat gaagaaagtg 541 atattttcat gttaattttt gatgccatgc actcatttcc agccaatgat gaagtccaga 601 aacttggatg caaagcttta catgtgctgt ttgagagagt ctcagaggag caactgactg 661 aatttgttga gaacaaagat tatatgatat tgttaagtgc gttaacaaat tttaaagatg 721 aagaggaaat tgtgcttcat gtgctgcatt gtttacattc cctagcgatt ccttgcaata 781 atgtggaagt cctcatgagt ggcaatgtca ggtgttataa tattgtggtg gaagctatga 841 aagcattccc tatgagtgaa agaattcaag aagtgagttg ctgtttgctc cataggctta 901 cattaggtaa ttttttcaat atcctggtat taaacgaagt ccatgagttt gtggtgaaag 961 ctgtgcagca gtacccagag aatgcagcat tgcagatctc agcgctcagc tgtttggccc 1021 tcctcactga gactattttc ttaaatcaag atttagagga aaagaatgag aatcaagaga 1081 atgatgatga gggggaagaa gataaattgt tttggctgga agcctgttac aaagcattaa 1141 cgtggcatag aaagaacaag cacgtgcagg aggccgcatg ctgggcacta aataatctcc 1201 ttatgtacca aaacagttta catgagaaga ttggagatga agatggccat ttcccagctc 1261 atagggaagt gatgctctcc atgctgatgc attcttcatc aaaggaagtt ttccaggcat 1321 ctgcgaatgc attgtcaact ctcttagaac aaaatgttaa tttcagaaaa atactgttat 1381 caaaaggaat acacctgaat gttttggagt taatgcagaa gcatatacat tctcctgaag 1441 tggctgaaag tggctgtaaa atgctaaatc atctttttga aggaagcaac acttccctgg 1501 atataatggc agcagtggtc cccaaaatac taacagttat gaaacgtcat gagacatcat 1561 taccagtgca gctggaggcg cttcgagcta ttttacattt tatagtgcct ggcatgccag 1621 aagaatccag ggaggataca gaatttcatc ataagctaaa tatggttaaa aaacagtgtt 1681 tcaagaatga tattcacaaa ctggtcctag cagctttgaa caggttcatt ggaaatcctg 1741 ggattcagaa atgtggatta aaagtaattt cttctattgt acattttcct gatgcattag 1801 agatgttatc cctggaaggt gctatggatt cagtgcttca cacactgcag atgtatccag 1861 atgaccaaga aattcagtgt ctgggtttaa gtcttatagg atacttgatt acaaagaaga 1921 atgtgttcat aggaactgga catctgctgg caaaaattct ggtttccagc ttataccgat 1981 ttaaggatgt tgctgaaata cagactaaag gatttcagac aatcttagca atcctcaaat 2041 tgtcagcatc tttttctaag ctgctggtgc atcattcatt tgacttagta atattccatc 2101 aaatgtcttc caatatcatg gaacaaaagg atcaacagtt tctaaacctc tgttgcaagt 2161 gttttgcaaa agtagctatg gatgattact taaaaaatgt gatgctagag agagcgtgtg 2221 atcagaataa cagcatcatg gttgaatgct tgcttctatt gggagcagat gccaatcaag 2281 caaaggaggg atcttcttta atttgtcagg tatgtgagaa agagagcagt cccaaattgg 2341 tggaactctt actgaatagt ggatctcgtg aacaagatgt acgaaaagcg ttgacgataa 2401 gcattgggaa aggtgacagc cagatcatca gcttgctctt aaggaggctg gccctggatg 2461 tggccaacaa tagcatttgc cttggaggat tttgtatagg aaaagttgaa ccttcttggc 2521 ttggtccttt atttccagat aagacttcta atttaaggaa acaaacaaat atagcatcta 2581 cactagcaag aatggtgatc agatatcaga tgaaaagtgc tgtggaagaa ggaacagcct 2641 caggcagcga tggaaatttt tctgaagatg tgctgtctaa atttgatgaa tggaccttta 2701 ttcctgactc ttctatggac agtgtgtttg ctcaaagtga tgacctggat agtgaaggaa 2761 gtgaaggctc atttcttgtg aaaaagaaat ctaattcaat tagtgtagga gaattttacc 2821 gagatgccgt attacagcgt tgctcaccaa atttgcaaag acattccaat tccttggggc 2881 ccatttttga tcatgaagat ttactgaagc gaaaaagaaa aatattatct tcagatgatt 2941 cactcaggtc atcaaaactt caatcccata tgaggcattc agacagcatt tcttctctgg 3001 cttctgagag agaatatatt acatcactag acctttcagc aaatgaacta agagatattg 3061 atgccctaag ccagaaatgc tgtataagtg ttcatttgga gcatcttgaa aagctggagc 3121 ttcaccagaa tgcactcacg agctttccac aacagctatg tgaaactctg aagagtttga 3181 cacatttgga cttgcacagt aataaattta catcatttcc ttcttatttg ttgaaaatga 3241 gttgtattgc taatcttgat gtctctcgaa atgacattgg accctcagtg gttttagatc 3301 ctacagtgaa atgtccaact ctgaaacagt ttaacctgtc atataaccag ctgtcttttg 3361 tacctgagaa cctcactgat gtggtagaga aactggagca gctcatttta gaaggaaata 3421 aaatatcagg gatatgctcc cccttgagac tgaaggaact gaagatttta aaccttagta 3481 agaaccacat ttcatcccta tcagagaact ttcttgaggc ttgtcctaaa gtggagagtt 3541 tcagtgccag aatgaatttt cttgctgcta tgcctttctt gcctccttct atgacaatcc 3601 taaaattatc tcagaacaaa ttttcctgta ttccagaagc aattttaaat cttccacact 3661 tgcggtcttt agatatgagc agcaatgata ttcagtacct accaggtccc gcacactgga 3721 aatctttgaa cttaagggaa ctcttattta gccataatca gatcagcatc ttggacttga 3781 gtgaaaaagc atatttatgg tctagagtag agaaactgca tctttctcac aataaactga 3841 aagagattcc tcctgagatt ggctgtcttg aaaatctgac atctctggat gtcagttaca 3901 acttggaact aagatccttt cccaatgaaa tggggaaatt aagcaaaata tgggatcttc 3961 ctttggatga actgcatctt aactttgatt ttaaacatat aggatgtaaa gccaaagaca 4021 tcataaggtt tcttcaacag cgattaaaaa aggctgtgcc ttataaccga atgaaactta 4081 tgattgtggg aaatactggg agtggtaaaa ccaccttatt gcagcaatta atgaaaacca 4141 agaaatcaga tcttggaatg caaagtgcca cagttggcat agatgtgaaa gactggccta 4201 tccaaataag agacaaaaga aagagagatc tcgtcctaaa tgtgtgggat tttgcaggtc 4261 gtgaggaatt ctatagtact catccccatt ttatgacgca gcgagcattg taccttgctg 4321 tctatgacct cagcaaggga caggctgaag ttgatgccat gaagccttgg ctcttcaata 4381 taaaggctcg cgcttcttct tcccctgtga ttctcgttgg cacacatttg gatgtttctg 4441 atgagaagca acgcaaagcc tgcatgagta aaatcaccaa ggaactcctg aataagcgag 4501 ggttccctgc catacgagat taccactttg tgaatgccac cgaggaatct gatgctttgg 4561 caaaacttcg gaaaaccatc ataaacgaga gccttaattt caagatccga gatcagcttg 4621 ttgttggaca gctgattcca gactgctatg tagaacttga aaaaatcatt ttatcggagc 4681 gtaaaaatgt gccaattgaa tttcccgtaa ttgaccggaa acgattatta caactagtga 4741 gagaaaatca gctgcagtta gatgaaaatg agcttcctca cgcagttcac tttctaaatg 4801 aatcaggagt ccttcttcat tttcaagacc cagcactgca gttaagtgac ttgtactttg 4861 tggaacccaa gtggctttgt aaaatcatgg cacagatttt gacagtgaaa gtggaaggtt 4921 gtccaaaaca ccctaaggga attatttcgc gtagagatgt ggaaaaattt ctttcaaaga 4981 aaaggaaatt tccaaagaac tacatgacac agtattttaa gctcctagaa aaattccaga 5041 ttgctttgcc aataggagaa gaatatttgc tggttccaag cagtttgtct gaccacaggc 5101 ctgtgataga gcttccccat tgtgagaact ctgaaattat catccgacta tatgaaatgc 5161 cttattttcc aatgggattt tggtcaagat taatcaatcg attacttgag atttcacctt 5221 acatgctttc agggagagaa cgagcacttc gcccaaacag aatgtattgg cgacaaggca 5281 tttacttaaa ttggtctcct gaagcttatt gtctggtagg atctgaagtc ttagacaatc 5341 atccagagag tttcttaaaa attacagttc cttcttgtag aaaaggctgt attcttttgg 5401 gccaagttgt ggaccacatt gattctctca tggaagaatg gtttcctggg ttgctggaga 5461 ttgatatttg tggtgaagga gaaactctgt tgaagaaatg ggcattatat agttttaatg 5521 atggcgaaga acatcaaaaa atcttacttg atgacttgat gaagaaagca gaggaaggag 5581 atctcttagt aaatccagat caaccaaggc tcaccattcc aatatctcag attgcccctg 5641 acttgatttt ggctgacctg cctagaaata ttatgttgaa taatgatgag ttggaatttg 5701 aacaagctcc agagtttctc ctaggtgatg gcagttttgg atcagtttac cgagcagcct 5761 atgaaggaga agaagtggct gtgaagattt ttaataaaca tacatcactc aggctgttaa 5821 gacaagagct tgtggtgctt tgccacctcc accaccccag tttgatatct ttgctggcag 5881 ctgggattcg tccccggatg ttggtgatgg agttagcctc caagggttcc ttggatcgcc 5941 tgcttcagca ggacaaagcc agcctcacta gaaccctaca gcacaggatt gcactccacg 6001 tagctgatgg tttgagatac ctccactcag ccatgattat ataccgagac ctgaaacccc 6061 acaatgtgct gcttttcaca ctgtatccca atgctgccat cattgcaaag attgctgact 6121 acggcattgc tcagtactgc tgtagaatgg ggataaaaac atcagagggc acaccagggt 6181 ttcgtgcacc tgaagttgcc agaggaaatg tcatttataa ccaacaggct gatgtttatt 6241 catttggttt actactctat gacattttga caactggagg tagaatagta gagggtttga 6301 agtttccaaa tgagtttgat gaattagaaa tacaaggaaa attacctgat ccagttaaag 6361 aatatggttg tgccccatgg cctatggttg aaaaattaat taaacagtgt ttgaaagaaa 6421 atcctcaaga aaggcctact tctgcccagg tctttgacat tttgaattca gctgaattag 6481 tctgtctgac gagacgcatt ttattaccta aaaacgtaat tgttgaatgc atggttgcta 6541 cacatcacaa cagcaggaat gcaagcattt ggctgggctg tgggcacacc gacagaggac 6601 agctctcatt tcttgactta aatactgaag gatacacttc tgaggaagtt gctgatagta 6661 gaatattgtg cttagccttg gtgcatcttc ctgttgaaaa ggaaagctgg attgtgtctg 6721 ggacacagtc tggtactctc ctggtcatca ataccgaaga tgggaaaaag agacataccc 6781 tagaaaagat gactgattct gtcacttgtt tgtattgcaa ttccttttcc aagcaaagca 6841 aacaaaaaaa ttttcttttg gttggaaccg ctgatggcaa gttagcaatt tttgaagata 6901 agactgttaa gcttaaagga gctgctcctt tgaagatact aaatatagga aatgtcagta 6961 ctccattgat gtgtttgagt gaatccacaa attcaacgga aagaaatgta atgtggggag 7021 gatgtggcac aaagattttc tccttttcta atgatttcac cattcagaaa ctcattgaga 7081 caagaacaag ccaactgttt tcttatgcag ctgtcagtga ttccaacatc ataacagtgg 7141 tggtagacac tgctctctat attgctaagc aaaatagccc tgttgtggaa gtgtgggata 7201 agaaaactga aaaactctgt ggactaatag actgcgtgca ctttttaagg gaggtaacgg 7261 taaaagaaaa caaggaatca aaacacaaaa tgtcttattc tgggagagtg aaaaccctct 7321 gccTTCAGAA GAACACTTCT CTTTGGATAG GAACTGGAGG AGGCCATATT TTACTCCTGG 7381 ATCTTTCAAC TCGTCGACTT ATACGTGTAA TTTACAACTT TTGTAATTCG GTCAGAGTCA 7441 TGATGACAGC ACAGCTAGGA AGCCTTAAAA ATGTCATGCT GGTATTGGGC TACAACCGGA 7501 AAAATACTGA AGGTACACAA AAGCAGAAAG AGATACAATC TTGCTTGACC GTTTGGGACA 7561 TCAATCTTCC ACATGAAGTG CAAAATTTAG AAAAACACAT TGAAGTGAGA AAAGAATTAG 7621 CTGAAAAAAT GAGACGAACA TCTGTTGAGT TGCCAACTTT CTTGTACAAA GTGGTTGATA 7681 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 7741 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACACT 7801 GGGATAATCT TGTACGCTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 7861 tgaaagatt