Transcript: Mouse NM_025861.3

Mus musculus PQ loop repeat containing 1 (Pqlc1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Pqlc1 (66943)
Length:
1989
CDS:
233..1048

Additional Resources:

NCBI RefSeq record:
NM_025861.3
NBCI Gene record:
Pqlc1 (66943)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025861.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000197583 CATAGTCATGATCCTTACGAT pLKO.1 475 CDS 100% 3.000 4.200 N Pqlc1 n/a
2 TRCN0000197913 GCTCATCTTGAGATCATGTTA pLKO.1 1581 3UTR 100% 5.625 3.938 N Pqlc1 n/a
3 TRCN0000200254 CTTCAAGACGGCCTACTTCTT pLKO.1 877 CDS 100% 4.950 3.465 N Pqlc1 n/a
4 TRCN0000182654 GCCCTTGTATGCCATAGCTAT pLKO.1 1491 3UTR 100% 4.950 3.465 N Pqlc1 n/a
5 TRCN0000178556 CAGAGCATAGTCATGATCCTT pLKO.1 470 CDS 100% 3.000 2.100 N Pqlc1 n/a
6 TRCN0000156320 CTACATCACCTACCTGTCCAT pLKO.1 706 CDS 100% 2.640 1.848 N SLC66A2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025861.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04177 pDONR223 100% 85.6% 88.9% None (many diffs) n/a
2 ccsbBroad304_04177 pLX_304 0% 85.6% 88.9% V5 (many diffs) n/a
3 TRCN0000479482 GGTCTCTGCTCCGCCTTTGCGGCG pLX_317 38.9% 85.6% 88.9% V5 (many diffs) n/a
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