Transcript: Mouse NM_026831.1

Mus musculus myosin binding protein H-like (Mybphl), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Mybphl (68753)
Length:
1287
CDS:
87..1154

Additional Resources:

NCBI RefSeq record:
NM_026831.1
NBCI Gene record:
Mybphl (68753)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_026831.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000089762 CCACAAGATACGGGCAATACA pLKO.1 594 CDS 100% 5.625 7.875 N Mybphl n/a
2 TRCN0000089760 GTATTAAGTTGGTGGACGTTT pLKO.1 541 CDS 100% 4.950 3.960 N Mybphl n/a
3 TRCN0000089759 CTCCACAAGATACGGGCAATA pLKO.1 592 CDS 100% 10.800 7.560 N Mybphl n/a
4 TRCN0000090638 GCCCAAGATCATCTGGCTAAA pLKO.1 956 CDS 100% 10.800 7.560 N Mybphl n/a
5 TRCN0000089758 CCCTCCTCAGAGTATTAAGTT pLKO.1 530 CDS 100% 5.625 3.938 N Mybphl n/a
6 TRCN0000090640 CTACAGTCACTGGCTATGATA pLKO.1 901 CDS 100% 5.625 3.938 N Mybphl n/a
7 TRCN0000117041 GAAAGCAGCTACTGTTTACAA pLKO.1 812 CDS 100% 5.625 3.938 N MYBPHL n/a
8 TRCN0000090641 CCCTAGAAATCCGCAAGCCTA pLKO.1 1039 CDS 100% 2.640 1.848 N Mybphl n/a
9 TRCN0000090642 CCTGGGATACACGGTGCAGAA pLKO.1 620 CDS 100% 1.350 0.945 N Mybphl n/a
10 TRCN0000090639 CACCATCAATATCCTGGTGAT pLKO.1 497 CDS 100% 0.405 0.284 N Mybphl n/a
11 TRCN0000089761 CAAGATCATCTGGCTAAAGAA pLKO.1 959 CDS 100% 5.625 3.375 N Mybphl n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_026831.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10046 pDONR223 100% 85.1% 82.8% None (many diffs) n/a
2 ccsbBroad304_10046 pLX_304 0% 85.1% 82.8% V5 (many diffs) n/a
3 TRCN0000481520 CCAATCGCTGTGAGAAACAAAACT pLX_317 33.5% 85.1% 82.8% V5 (many diffs) n/a
4 ccsbBroadEn_10045 pDONR223 100% 84.9% 82.8% None (many diffs) n/a
5 ccsbBroad304_10045 pLX_304 0% 84.9% 82.8% V5 (many diffs) n/a
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