Transcript: Mouse NM_027116.1

Mus musculus NTPase, KAP family P-loop domain containing 1 (Nkpd1), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Nkpd1 (69547)
Length:
2753
CDS:
1..2478

Additional Resources:

NCBI RefSeq record:
NM_027116.1
NBCI Gene record:
Nkpd1 (69547)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_027116.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000346422 TACCCTTCTCCGTGCCGATTA pLKO_005 1583 CDS 100% 0.000 0.000 N Nkpd1 n/a
2 TRCN0000346348 CCGATGGCCTAAGTCCTTAAA pLKO_005 2467 CDS 100% 13.200 10.560 N Nkpd1 n/a
3 TRCN0000346423 GCGCTTTCTAGGCACTGATTT pLKO_005 2187 CDS 100% 13.200 9.240 N Nkpd1 n/a
4 TRCN0000353276 GGTGGAACTGCTCACGGATTT pLKO_005 1308 CDS 100% 10.800 7.560 N Nkpd1 n/a
5 TRCN0000346349 ACATCCCTCCTGACCTGATGA pLKO_005 2549 3UTR 100% 4.950 3.465 N Nkpd1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_027116.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14462 pDONR223 100% 60% 44.9% None (many diffs) n/a
2 ccsbBroad304_14462 pLX_304 0% 60% 44.9% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000475284 CAGGAACTCGGGGGAGTCCCCGTC pLX_317 15.6% 60% 44.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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