Construct: ORF TRCN0000475284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009016.1_s317c1
- Derived from:
- ccsbBroadEn_14462
- DNA Barcode:
- CAGGAACTCGGGGGAGTCCCCGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NKPD1 (284353)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475284
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284353 | NKPD1 | NTPase KAP family P-loop do... | XM_011526805.2 | 92.6% | 64.3% | 1_141del;1383_1385delGCAinsNN |
| 2 | human | 284353 | NKPD1 | NTPase KAP family P-loop do... | XM_011526804.2 | 85.4% | 59.3% | 1_309del;1551_1553delGCAinsNN |
| 3 | human | 284353 | NKPD1 | NTPase KAP family P-loop do... | NM_198478.3 | 73.1% | 50.8% | 1_666del;1908_1910delGCAinsNN |
| 4 | human | 284353 | NKPD1 | NTPase KAP family P-loop do... | XM_011526802.2 | 73.1% | 50.8% | 1_666del;1908_1910delGCAinsNN |
| 5 | human | 284353 | NKPD1 | NTPase KAP family P-loop do... | XM_011526799.2 | 70.8% | 49.2% | 1_747del;1989_1991delGCAinsNN |
| 6 | mouse | 69547 | Nkpd1 | NTPase, KAP family P-loop d... | NM_027116.1 | 60% | 44.9% | (many diffs) |
| 7 | mouse | 69547 | Nkpd1 | NTPase, KAP family P-loop d... | XM_006540350.2 | 50.4% | 37.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1401
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagcaggag gccgcgcagc gcgagagcga ggagctgcag cacgtgcagt 121 ggcggccgcg tgccgtgagc ggctggggcg tcccgcagct actgtggtac ctggtgttcc 181 tgcagcccat catcaccgag gtgcacctgc ggcgcaggaa cgtgcagttc cttttcatcc 241 gctttagcgc ctggcagtac gcgggcaccg acaagctgtg ggccggcctg gtgaccacgt 301 tgtgcgaggg catccgccgc cactatggcg cactgccctt cagcgtgtac tcggtgctgg 361 gcaacaagcc ggccaccagg caggactgct gccagagcga gtggcattgt cggcgccgcg 421 tgtgcctggg gctgctggcg ctgctggcgg cgctgggcct gggtgtgggg ctgctctact 481 tgtcactggg cggccacgcg ctgggccacg gcagcccgag cggcagcctg ctcaaggtgt 541 ttggcggcgc ggccaccaca ctgtcgggct cggggctgct catggccgtg tactcggtgg 601 gcaagcacct gttcgtaagc cagcgcaaga agatcgagcg gctggtgtcg cgtgaaaagt 661 tcggcagcca gctgggtttc atgtgcgagg tgaagaagga ggtggagctg ctcaccgact 721 tcctgtgctt cctggagatc taccagcggc gcaggctgcg cgtggtgctg gaggtcaccg 781 ggctggacac gtgctacccg gagcgcgtgg tgggcgtgct caacgccatc aacacgctgc 841 tgtccgacag ccacgcgccc ttcatcttca tcctggtcgt ggaccccagc atcctggccg 901 cgtgcctaga gagcgcgggc aacatgaagg gcacggccga taacggctac ctcttcctca 961 accgcactgt cacgctgccc ttctctgtgc ccattatggg ccgccgcacc aagctgcagt 1021 tcctgcacga tgcggtgcag agccgcgacg acctgttgta ccgcgagatg acgcgcaagc 1081 cgtggctgcc gggggacgcc gggggcgaga gcgcgcagct gctggcggtg caggcgcagg 1141 cggggacgga gcgcgggcag ggccgcatcg acgacgaggc ggcgcggcga atccaggagg 1201 cgctcttctg ccttcacgac gagcgcgact gcctctacga gtacgtgccc gacaacgtgg 1261 tgtccatgcg gcgcatcgtc aacaccgtgc ccatcaccgt gcgcctgctn ngcagcagca 1321 gcagcagggg gactttgggg gccccacgcc gcgccaggcg gtggcgtggg tggtgctcgc 1381 caaccagtgg ccgtgccgcc tgagctgggc gctgcagtgc ctggaggacc ggcagcagac 1441 cgggggcgcg cccgagggcc gcgcgcgcct ctgggacgtt ttccgcgaca acagccgcga 1501 gctgcacacc atgaccaagg cgttgcagaa cgtgctcgac ctggacggcg accccgagct 1561 cttcgagcgc ttcctgggcg ccgacttccc cttcaccgtg gccgaggcgc agagcctgcT 1621 GCGCTGCACG GTCAACCTGG ACCACTCCAT CCGCCGGCGC ATGGGTCTCA TCCGAGCCGT 1681 CAGCGCGCTC AAGCCGCCCA GCCCGCCCAA GTCCCCTACC CGCGATACCC CCCACGCTGC 1741 CCACCGGGCC AACAGCGCCT CCAGGGCGCC CCCGTCGGGC CGTGCCTCAG GGCAAGCCGG 1801 CGAAGGCCAC CACACTGGGG ACTTGGCCCA CAGGGGCAAG CTATGGCCGG TGGCCTGTGC 1861 GCTCTTCCGT CCAGGGCAAT CCAGCCCAGG TGGGCCTTTG CCAACTTTCT TGTACAAAGT 1921 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 1981 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 2041 CGACAGGAAC TCGGGGGAGT CCCCGTCACG CGTTAAGTCg acaatcaacc tctggattac 2101 aaaatttgtg aaagatt