Transcript: Mouse NM_027534.2

Mus musculus 3-ketodihydrosphingosine reductase (Kdsr), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Kdsr (70750)
Length:
5582
CDS:
92..1090

Additional Resources:

NCBI RefSeq record:
NM_027534.2
NBCI Gene record:
Kdsr (70750)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_027534.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000251232 ATAACTCTGGTTGCACGAAAT pLKO_005 266 CDS 100% 10.800 15.120 N Kdsr n/a
2 TRCN0000251230 ACCTTGGAAGTTTCGATAATA pLKO_005 1011 CDS 100% 15.000 12.000 N Kdsr n/a
3 TRCN0000251228 GACATCTGACAGTACAATTAA pLKO_005 3257 3UTR 100% 15.000 10.500 N Kdsr n/a
4 TRCN0000251229 TGATGTGTCTCAAGACTATAA pLKO_005 367 CDS 100% 13.200 9.240 N Kdsr n/a
5 TRCN0000251231 CCTACTCTTCATCCAAGTTTG pLKO_005 645 CDS 100% 10.800 7.560 N Kdsr n/a
6 TRCN0000054408 ACGCCTTTAATCCCAGCACTT pLKO.1 2520 3UTR 100% 4.050 2.025 Y Mtif2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_027534.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06231 pDONR223 100% 87.8% 92.7% None (many diffs) n/a
2 ccsbBroad304_06231 pLX_304 0% 87.8% 92.7% V5 (many diffs) n/a
3 TRCN0000468609 GAGCCATCAAATCGACTAGCCAAG pLX_317 37.4% 87.8% 92.7% V5 (many diffs) n/a
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