Construct: ORF TRCN0000468609
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001427.1_s317c1
- Derived from:
- ccsbBroadEn_06231
- DNA Barcode:
- GAGCCATCAAATCGACTAGCCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KDSR (2531)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468609
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2531 | KDSR | 3-ketodihydrosphingosine re... | NM_002035.4 | 99.8% | 100% | 261G>A |
| 2 | human | 2531 | KDSR | 3-ketodihydrosphingosine re... | XM_005266677.3 | 89.6% | 89.7% | 261G>A;777_778ins102 |
| 3 | human | 2531 | KDSR | 3-ketodihydrosphingosine re... | XM_017025690.2 | 76.7% | 76.8% | 0_1ins231;30G>A |
| 4 | mouse | 70750 | Kdsr | 3-ketodihydrosphingosine re... | NM_027534.2 | 87.8% | 92.7% | (many diffs) |
| 5 | mouse | 70750 | Kdsr | 3-ketodihydrosphingosine re... | NM_001316750.1 | 70.2% | 72.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1062
- ORF length:
- 996
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gctgctggct gccgccttcc tcgtggcctt cgtgctgctg ctgtacatgg 121 tgtctccgct catcagcccc aagcccctcg ccctgcccgg ggcgcatgtg gtggttacag 181 gaggttccag tggcatcggg aagtgcattg ctatcgagtg ctataaacaa ggagctttta 241 taactctggt tgcacgaaat gaggataagc tgctgcaggc aaagaaagaa attgaaatgc 301 actctattaa tgacaaacag gtggtacttt gcatatcagt tgatgtatct caagactata 361 accaagtaga gaatgtcata aaacaagcac aggagaaact gggtccagtg gacatgctgg 421 taaattgtgc aggaatggca gtgtcaggaa aatttgaaga tcttgaagtt agtacctttg 481 aaaggttaat gagcatcaat tacctgggca gcgtgtaccc cagccgggcc gtgatcacca 541 ccatgaagga gcgccgggtg ggcaggatcg tgtttgtgtc ctcccaggca ggacagttgg 601 gattattcgg tttcacagcc tactctgcat ccaagtttgc cataagggga ttggcagaag 661 ctttgcagat ggaggtgaag ccatataatg tcTACATCAC AGTTGCTTAC CCACCAGACA 721 CAGACACACC TGGCTTTGCC GAAGAAAACA GAACAAAGCC TTTGGAGACT CGACTTATTT 781 CAGAGACCAC ATCTGTGTGC AAACCAGAAC AGGTGGCCAA ACAAATTGTT AAAGATGCCA 841 TACAAGGAAA TTTCAACAGT TCCCTTGGCT CAGATGGGTA CATGCTCTCG GCCCTGACCT 901 GTGGGATGGC TCCAGTAACT TCTATTACTG AGGGGCTCCA GCAGGTGGTC ACCATGGGCC 961 TTTTCCGCAC TATTGCTTTG TTTTACCTTG GAAGTTTTGA CAGCATAGTT CGTCGCTGCA 1021 TGATGCAGAG AGAAAAATCT GAAAATGCAG ACAAAACTGC CTACCCAACT TTCTTGTACA 1081 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1141 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1201 AGGACGAGAG CCATCAAATC GACTAGCCAA GACGCGTTAA GTCgacaatc aacctctgga 1261 ttacaaaatt tgtgaaagat t