Transcript: Human NM_139158.2

Homo sapiens cyclin dependent kinase 15 (CDK15), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-05
Taxon:
Homo sapiens (human)
Gene:
CDK15 (65061)
Length:
3587
CDS:
150..1304

Additional Resources:

NCBI RefSeq record:
NM_139158.2
NBCI Gene record:
CDK15 (65061)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001144734 TGGATGCTGAGACATATACT pXPR_003 GGG 409 35% 6 1.2749 CDK15 CDK15 76486
2 BRDN0001146647 CTCCAAGTTCAAGTAAGATG pXPR_003 AGG 151 13% 3 0.1612 CDK15 CDK15 76485
3 BRDN0001145059 GGCTCCCAGCAAAGCATCAG pXPR_003 GGG 652 56% 8 0.1432 CDK15 CDK15 76483
4 BRDN0001148122 AAACAATCACTGTTACTCCG pXPR_003 TGG 69 6% 2 0.1326 CDK15 CDK15 76484
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_139158.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000002098 AGGCTCTTATGCGACAGTTTA pLKO.1 329 CDS 100% 13.200 18.480 N CDK15 n/a
2 TRCN0000196597 GAAGCACTTGTTCATGATTAT pLKO.1 1134 CDS 100% 13.200 9.240 N CDK15 n/a
3 TRCN0000199747 GAGGGCTTCATCCTCATAATG pLKO.1 577 CDS 100% 13.200 9.240 N CDK15 n/a
4 TRCN0000196498 GCTTACTAAGAAGCTTCAAAT pLKO.1 1375 3UTR 100% 13.200 9.240 N CDK15 n/a
5 TRCN0000199688 GTTTCAGGAGTGAGGCTAAAG pLKO.1 1212 CDS 100% 10.800 7.560 N CDK15 n/a
6 TRCN0000002100 AGCAGAATAAATGGACAACTA pLKO.1 360 CDS 100% 4.950 3.465 N CDK15 n/a
7 TRCN0000002099 CAAATCTAACTCCATACTGAA pLKO.1 1391 3UTR 100% 4.950 3.465 N CDK15 n/a
8 TRCN0000002096 CACCAAAGAGACACTGACATT pLKO.1 503 CDS 100% 4.950 3.465 N CDK15 n/a
9 TRCN0000002097 CTACCTAACTACAATCCAGAA pLKO.1 987 CDS 100% 4.050 2.835 N CDK15 n/a
10 TRCN0000199929 GCCACTGAATATTCCTCTGAG pLKO.1 816 CDS 100% 4.050 2.835 N CDK15 n/a
11 TRCN0000166364 CACACACACACACACACACAA pLKO.1 2111 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_139158.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12510 pDONR223 100% 90.8% 90.8% None 1048_1152del n/a
2 ccsbBroad304_12510 pLX_304 0% 90.8% 90.8% V5 1048_1152del n/a
3 TRCN0000467466 AAAGGGTTGCCTAGCCCCCAAGGT pLX_317 7.9% 90.8% 90.8% V5 1048_1152del n/a
4 ccsbBroadEn_15139 pDONR223 0% 90.8% 90.8% None 1048_1152del n/a
5 ccsbBroad304_15139 pLX_304 0% 90.8% 90.8% V5 1048_1152del n/a
6 TRCN0000480211 AGCAATTCTAAGCAATCTCCAACG pLX_317 35.3% 90.8% 90.8% V5 1048_1152del n/a
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