Transcript: Mouse NM_172871.2

Mus musculus kelch-like 9 (Klhl9), mRNA.

Source:
NCBI, updated 2017-04-22
Taxon:
Mus musculus (mouse)
Gene:
Klhl9 (242521)
Length:
4217
CDS:
507..2360

Additional Resources:

NCBI RefSeq record:
NM_172871.2
NBCI Gene record:
Klhl9 (242521)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_172871.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000242026 CACGCACAGTTCGGTTGTATT pLKO_005 596 CDS 100% 13.200 18.480 N Klhl9 n/a
2 TRCN0000335938 TGCTAACACCTACAATCTTAT pLKO_005 986 CDS 100% 13.200 18.480 N KLHL9 n/a
3 TRCN0000174969 GCTAGTTGTCAGTAAAGAGTT pLKO.1 1436 CDS 100% 4.950 6.930 N Klhl9 n/a
4 TRCN0000242027 TCGATCTGATTCTACTCATTT pLKO_005 1385 CDS 100% 13.200 10.560 N Klhl9 n/a
5 TRCN0000216127 CAGATTTGATCCTCGTTATAA pLKO.1 1616 CDS 100% 15.000 10.500 N Klhl9 n/a
6 TRCN0000242030 CAGATTTGATCCTCGTTATAA pLKO_005 1616 CDS 100% 15.000 10.500 N Klhl9 n/a
7 TRCN0000175213 CCCTGTTCTAACCTAATATAA pLKO.1 2702 3UTR 100% 15.000 10.500 N Klhl9 n/a
8 TRCN0000242028 CCCTGTTCTAACCTAATATAA pLKO_005 2702 3UTR 100% 15.000 10.500 N Klhl9 n/a
9 TRCN0000369065 CTATGTTGTTGGTGGATATTC pLKO_005 2147 CDS 100% 13.200 9.240 N KLHL9 n/a
10 TRCN0000242029 GAAGTGGATAAATACGTAAAT pLKO_005 1008 CDS 100% 13.200 9.240 N Klhl9 n/a
11 TRCN0000005852 GTCCAGAAATATGACCCAGAA pLKO.1 2196 CDS 100% 4.050 2.835 N KLHL9 n/a
12 TRCN0000175414 CCTTTGTGCTTTCCAGCAATA pLKO.1 1102 CDS 100% 10.800 6.480 N Klhl9 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_172871.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08613 pDONR223 100% 91.5% 98.7% None (many diffs) n/a
2 ccsbBroad304_08613 pLX_304 0% 91.5% 98.7% V5 (many diffs) n/a
3 TRCN0000480620 CGGATATTAATTAGCAACCTAAAC pLX_317 19.4% 91.5% 98.7% V5 (many diffs) n/a
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