Construct: ORF TRCN0000480620

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000276.1_s317c1
Derived from:
ccsbBroadEn_08613
DNA Barcode:
CGGATATTAATTAGCAACCTAAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KLHL9 (55958)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480620
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55958 KLHL9 kelch like family member 9 NM_018847.4 99.9% 100% 459C>T
2 human 90293 KLHL13 kelch like family member 13 NM_001168303.1 86.5% 92.2% (many diffs)
3 human 90293 KLHL13 kelch like family member 13 NM_001168301.1 83% 88.4% (many diffs)
4 human 90293 KLHL13 kelch like family member 13 NM_001168302.1 83% 88.4% (many diffs)
5 human 90293 KLHL13 kelch like family member 13 XM_017029950.1 83% 88.4% (many diffs)
6 human 90293 KLHL13 kelch like family member 13 NM_001168300.1 81.7% 87.1% (many diffs)
7 human 90293 KLHL13 kelch like family member 13 NM_033495.3 81% 86.3% (many diffs)
8 human 90293 KLHL13 kelch like family member 13 XM_011531411.1 81% 86.3% (many diffs)
9 human 90293 KLHL13 kelch like family member 13 NM_001168299.1 80.6% 85.9% (many diffs)
10 human 90293 KLHL13 kelch like family member 13 XM_011531409.3 80.2% 85.5% (many diffs)
11 human 90293 KLHL13 kelch like family member 13 XM_011531410.3 80.2% 85.5% (many diffs)
12 mouse 242521 Klhl9 kelch-like 9 NM_172871.2 91.5% 98.7% (many diffs)
13 mouse 67455 Klhl13 kelch-like 13 NM_026167.4 79.7% 85.6% (many diffs)
14 mouse 67455 Klhl13 kelch-like 13 XM_011247492.2 79.7% 85.6% (many diffs)
15 mouse 67455 Klhl13 kelch-like 13 XM_011247493.2 79.7% 85.6% (many diffs)
16 mouse 67455 Klhl13 kelch-like 13 XM_011247494.2 79.7% 85.6% (many diffs)
17 mouse 67455 Klhl13 kelch-like 13 XM_017318610.1 79.7% 85.6% (many diffs)
18 mouse 67455 Klhl13 kelch-like 13 NM_001310466.1 79.5% 85.4% (many diffs)
19 mouse 67455 Klhl13 kelch-like 13 NM_001290476.2 78.5% 84.3% (many diffs)
20 mouse 67455 Klhl13 kelch-like 13 XM_011247489.2 77.8% 83.6% (many diffs)
21 mouse 67455 Klhl13 kelch-like 13 XM_011247488.2 77.5% 83.2% (many diffs)
22 mouse 67455 Klhl13 kelch-like 13 XM_011247484.2 77.1% 82.8% (many diffs)
23 mouse 67455 Klhl13 kelch-like 13 XM_011247485.2 77.1% 82.8% (many diffs)
24 mouse 67455 Klhl13 kelch-like 13 XM_011247486.2 77.1% 82.8% (many diffs)
25 mouse 67455 Klhl13 kelch-like 13 XM_011247487.2 77.1% 82.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1920
ORF length:
1851
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaaagtgtcc cttggtaacg gcgaaatggg cgtctctgcc catttgcagc
121cttgtaaggc aggaaccaca cgctttttta ccagcaatac tcacagttcg gtggtattgc
181aaggctttga tcagcttaga atagaaggat tgctttgtga tgtgaccctg gtaccaggtg
241atggagatga aatcttccct gttcacagag ctatgatggc gtctgctagt gattatttca
301aagccatgtt cacaggtgga atgaaagaac aagatttgat gtgcattaag cttcatgggg
361tgaacaaggt tggtctgaag aaaatcattg attttattta tactgcaaaa ctttctctta
421atatggacaa tcttcaggac acacttgaag ctgctagctt tttacaaata ttacccgttt
481tggatttctg taaagtattt cttatatcag gagtctcttt ggataattgt gttgaggttg
541gacgaattgc taacacctac aatcttatag aagtggataa atatgttaat aatttcatcc
601tgaagaactt tcctgcttta ttgagtactg gggagtttct aaaactccct tttgaacgac
661ttgcatttgt gctttccagt aatagtctta agcactgtac cgaacttgaa ctctttaagg
721cagcctgtcg ctggctaagg ttggaagacc ctcggatgga ttatgctgca aagttaatga
781agaatattcg atttccactg atgacaccac aggatctcat caattacgtg cagacagtag
841atttcatgag aacagacaat acctgcgtga atttgctttt ggaagctagc aattaccaaa
901tgatgccata tatgcagcca gtgatgcagt cagatagaac tgccattcga tctgactcca
961ctcacttggt tacattagga ggagttttga ggcagcagct ggttgtcagt aaagaattac
1021ggatgtatga tgaaagggca caagaatgga gatctttagc cccaatggat gctccccgtt
1081accagcatgg tattgctgtc attggaaact ttctttatgt agttggtggt cagagtaatt
1141atgatacaaa aggaaaaact gctgttgata cagttttcag atttgatcct cggtataata
1201aatggatgca ggttgcatca ttaaatgaaa agcgcacatt ctttcacttg agtgccctca
1261aaggacattt gtatgcagtt ggtgggcgca gtgcagctgg tgaactggcc acagtagaat
1321gttacaaccc aagaatgaat gagtggagct atgttgcaaa aatgagtgaa ccccactatg
1381gtcatgctgg aacagtatat ggaggcttaa tgtatatttc aggaggaatt acccatgaca
1441ctttccaaaa tgagctcatg tgttttgacc cagatacaga taaatggatg caaaaggctc
1501caatgactac agtcagaggt ctgcattgca tgtgtacagt tggagataag ctctatgtca
1561ttggtgGCAA TCACTTCAGA GGAACAAGTG ATTATGATGA TGTTCTAAGC TGTGAATACT
1621ATTCACCAAC CCTTGACCAG TGGACCCCAA TTGCCGCCAT GTTAAGAGGC CAAAGTGATG
1681TTGGAGTTGC CGTCTTTGAA AATAAAATCT ATGTTGTTGG TGGATATTCT TGGAATAATC
1741GTTGTATGGT AGAAATTGTC CAGAAATATG ACCCAGAAAA AGATGAGTGG CATAAAGTTT
1801TTGATCTTCC AGAGTCACTT GGTGGCATTC GAGCCTGTAC ACTCACAGTT TTTCCACCTG
1861AAGAAAACCC TGGGTCACCT TCTAGAGAAT CACCTCTTTC AGCACCTTCA GATCATTCTT
1921TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1981GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
2041TTTATATATC TTGTGGAAAG GACGACGGAT ATTAATTAGC AACCTAAACA CGCGTTAAGT
2101Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)