Transcript: Mouse NM_175514.2

Mus musculus family with sequence similarity 171, member B (Fam171b), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Fam171b (241520)
Length:
3394
CDS:
22..2487

Additional Resources:

NCBI RefSeq record:
NM_175514.2
NBCI Gene record:
Fam171b (241520)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_175514.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000125289 GCTTAGAAACAACCTGATAAA pLKO.1 2913 3UTR 100% 13.200 18.480 N Fam171b n/a
2 TRCN0000125291 CCTCAGATTGGAGCCGATATT pLKO.1 1856 CDS 100% 13.200 9.240 N Fam171b n/a
3 TRCN0000125293 CACTCCTTTATAGACCTGAAA pLKO.1 2062 CDS 100% 4.950 3.465 N Fam171b n/a
4 TRCN0000125290 CCCTTCTACATACAAGTAATA pLKO.1 836 CDS 100% 13.200 7.920 N Fam171b n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_175514.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13341 pDONR223 100% 85.8% 88.2% None (many diffs) n/a
2 ccsbBroad304_13341 pLX_304 0% 85.8% 88.2% V5 (many diffs) n/a
3 TRCN0000466410 AGAACAACCTTTAAGGACCCCTCA pLX_317 16.5% 85.8% 88.2% V5 (many diffs) n/a
Download CSV