Construct: ORF TRCN0000466410
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000154.1_s317c1
- Derived from:
- ccsbBroadEn_13341
- DNA Barcode:
- AGAACAACCTTTAAGGACCCCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM171B (165215)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466410
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 165215 | FAM171B | family with sequence simila... | NM_177454.4 | 99.6% | 99.6% | (many diffs) |
| 2 | mouse | 241520 | Fam171b | family with sequence simila... | NM_175514.2 | 85.8% | 88.2% | (many diffs) |
| 3 | mouse | 241520 | Fam171b | family with sequence simila... | XM_006499465.2 | 77.5% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2550
- ORF length:
- 2484
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaggctctgc cggcgtgtcc cctgcaccct gcttctcggc ctggccgtgg 121 tgctgctgaa agcgcggctg gtccccgcgg ccgccagagc ggaactcagc cgctccgacc 181 tcagcctcat ccaacagcag cagcagcagc agcagcagca acaacaacaa caacagcaaa 241 agcagctgga ggaggctgag gaggagagga cagaggtgcc tggggcaacc tccaccttga 301 cggttccagt gtctgtattt atgttgaaag tccaggtgaa tgacatcatc agtcgtcagt 361 acctgagcca agcagttgta gaagtgtttg taaactacac gaagacaaat tccacagtaa 421 ctaaaagcaa tggagcagtg ctgataaaag taccctacaa attaggactt agtttaacta 481 ttattgctta caaagatggc tacgtgttga cccctctgcc ttggaaaacc agaagaatgc 541 caatatattc atcagttaca ctttcactgt tcccgcaaag ccaagcaaat atatggctat 601 ttgaagacac tgttttaatt actggaaaat tagctgatgc caagtctcaa ccaagtgttc 661 agttttcaaa agccttaatt aaacttcctg acaaccatca tattagcaac gttactggct 721 atcttacagt tctacaacag tttttgaaag tggacaattt tctgcataca actggaatta 781 ctctcaataa accaggtttt gaaaacattg aattgactcc tcttgctgca atatgtgtga 841 aaatatattc tggaggaaaa gaactaaagg tcaatggctc tattcaagtt tctcttcctc 901 ttctacgtct gaatgatata agtgcagggg atcgcatacc tgcttggaca tttgatatga 961 acacaggtgc ttggataaat catggtcggg gaatggtcaa ggaacataac aatcatttaa 1021 tctggacata tgatgcacca catttggggt actggatagc agctccactt ccaggaacta 1081 gaggttcagg tataaatgaa gattccaagg acataactgc ctaccacaca gtgtttctta 1141 cagccatatt aggaggaaca atagtcattg tcattggatt ttttgctgta ctactttgtt 1201 attgcaggga caagtgtggt actccacaga aaagagaaag aaatatcact aaacttgagg 1261 tcctcaagag agaccagaca acttcaacaa cacacataaa tcatatcagt acagttaaag 1321 ttgcattaaa agctgaggac aagtcgcagt tattcaatgc caaaaactcc tcatatagtc 1381 ctcagaaaaa ggaaccatca aaggcagaaa cagaagaaag agtttccatg gtaaaaactc 1441 gggacgattt taaaatctac aatgaagatg tttcatttct atcagtcaat caaaataatt 1501 actcaagaaa cccaacacag tctttggagc ccaatgtagg gtccaaacaa cctaaacata 1561 ttaacaacaa tctatcttca tctctaggtg atgctcaaga tgaaaagagg tatctcacag 1621 gtaatgagga ggcgtatggg cgttcccata ttcctgaaca gcttatgcat atttacagcc 1681 aacccattgc catccttcaa acatctgacc ttttctccac accggaacaa ttacatactg 1741 ctaagtcagc tactttgcca agaaagggac agttagtcta tggccaattg atggaaccag 1801 taaatcgaga gaactttacg cagaccttgc ccaaaatgcc aattcattcc catgcacagc 1861 ccccagatgc cagggaagag gatatcatac ttgaaggtca acagagcctg ccatcccagg 1921 cttcagattg gagccgatac tcaagcagct tactggaatc cgtctctgtt cctggaacac 1981 taaatgaggc tgttgtaatg actccatttt catcggaact tcaaggaatt tcagaacaga 2041 ccctcctgga gctgtccaaa ggaaagccct ccccgcatcc cagagcctgg tttgtgtctc 2101 ttgatggaaa gccagttgca caagtgaggc actcctttat agaccTGAAA AAGGGCAAGA 2161 GAACCCAGAG CAATGACACC AGTCTGGACT CTGGGGTGGA CATGAATGAG CTTCACTCAA 2221 GTAGAAAGCT CGAGAGGGAG AAAACATTCA TCAAAAGCAT GCATCAGCCC AAGATCCTTT 2281 ACTTAGAAGA TTTAGACCTA AGCAGCAGTG AGAGTGGAAC CACCGTCTGT TCCCCTGAAG 2341 ACCCAGCTTT AAGGCACATC CTAGATGGAG GGAGTGGAGT GATCATGGAG CACCCTGGAG 2401 AAGAGTCGCC AGGAAGGAAA AGCACTGTTG AAGATTTTGA AGCTAATACA TCCCCCACTA 2461 AAAGAAGGGG CAGACCACCA CTAGCCAAAA GAGATAGCAA GACTAACATC TGGAAGAAGC 2521 GAGAGGAACG CCCACTGATT CCCATAAATT ACCCAACTTT CTTGTACAAA GTGGTTGATA 2581 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2641 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGAAC 2701 AACCTTTAAG GACCCCTCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2761 tgaaagatt