Transcript: Human NM_178270.3

Homo sapiens autophagy related 4A cysteine peptidase (ATG4A), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-06-08
Taxon:
Homo sapiens (human)
Gene:
ATG4A (115201)
Length:
2142
CDS:
161..1171

Additional Resources:

NCBI RefSeq record:
NM_178270.3
NBCI Gene record:
ATG4A (115201)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_178270.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000416255 TGATATAAGTGCTCGTCTATG pLKO_005 301 CDS 100% 10.800 15.120 N ATG4A n/a
2 TRCN0000433463 TTGCTACTCTATCCATCAAAT pLKO_005 532 CDS 100% 13.200 10.560 N ATG4A n/a
3 TRCN0000073796 CAGATACAGATGAGCTGGTAT pLKO.1 228 CDS 100% 4.950 3.960 N ATG4A n/a
4 TRCN0000436397 GACAGGCCTCCCGATTCTTTA pLKO_005 752 CDS 100% 13.200 9.240 N ATG4A n/a
5 TRCN0000416712 TAGTCAGCAAGTGCCTGATAT pLKO_005 1249 3UTR 100% 13.200 9.240 N ATG4A n/a
6 TRCN0000432878 ACAGCGAATGAACATCCTAAA pLKO_005 892 CDS 100% 10.800 7.560 N ATG4A n/a
7 TRCN0000424537 GATGCTGGCTCAAGCCCTTAT pLKO_005 412 CDS 100% 10.800 7.560 N ATG4A n/a
8 TRCN0000073793 CCCGGAAAGAAATAGAACAAT pLKO.1 1365 3UTR 100% 5.625 3.938 N ATG4A n/a
9 TRCN0000073797 CCAGATACAGATGAGCTGGTA pLKO.1 227 CDS 100% 2.640 1.848 N ATG4A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178270.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04675 pDONR223 100% 84.4% 84.4% None 628_629ins186 n/a
2 ccsbBroad304_04675 pLX_304 0% 84.4% 84.4% V5 628_629ins186 n/a
3 TRCN0000470578 AACGAGCTAATCGACGCCTGCCAG pLX_317 40.4% 84.4% 84.4% V5 628_629ins186 n/a
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