Construct: ORF TRCN0000470578

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016388.1_s317c1
Derived from:
ccsbBroadEn_04675
DNA Barcode:
AACGAGCTAATCGACGCCTGCCAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATG4A (115201)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470578
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 115201 ATG4A autophagy related 4A cystei... NM_052936.5 100% 100%
2 human 115201 ATG4A autophagy related 4A cystei... NM_178270.3 84.4% 84.4% 628_629ins186
3 human 115201 ATG4A autophagy related 4A cystei... NM_001321287.1 80.6% 80.6% 0_1ins231
4 human 115201 ATG4A autophagy related 4A cystei... NM_001321288.1 80.6% 80.6% 0_1ins231
5 human 115201 ATG4A autophagy related 4A cystei... NM_178271.2 80.6% 80.6% 0_1ins231
6 human 115201 ATG4A autophagy related 4A cystei... XM_011530842.1 80.6% 80.6% 0_1ins231
7 human 115201 ATG4A autophagy related 4A cystei... NM_001321289.1 65% 65% 0_1ins231;397_398ins186
8 human 115201 ATG4A autophagy related 4A cystei... NM_001321290.1 56.7% 56.7% 0_1ins516
9 human 115201 ATG4A autophagy related 4A cystei... NR_135608.1 49% (many diffs)
10 mouse 666468 Atg4a autophagy related 4A, cyste... NM_174875.3 89.2% 91.7% (many diffs)
11 mouse 666468 Atg4a autophagy related 4A, cyste... XM_017318601.1 88.4% 90.7% (many diffs)
12 mouse 666468 Atg4a autophagy related 4A, cyste... XM_017318602.1 88.4% 90.7% (many diffs)
13 mouse 666468 Atg4a autophagy related 4A, cyste... XM_017318599.1 76.4% 78.5% (many diffs)
14 mouse 666468 Atg4a autophagy related 4A, cyste... XM_017318603.1 72% 74% (many diffs)
15 mouse 666468 Atg4a autophagy related 4A, cyste... XM_017318600.1 71.5% 73.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1260
ORF length:
1194
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gtcagtttta tccaagtatg aagatcagat tactattttc actgactacc
121tagaagaata tccagataca gatgagctgg tatggatctt agggaagcag catctcctta
181aaacagaaaa atctaagctg ttgtctgata taagtgctcg tctatggttt acatacagaa
241ggaaattttc accaattggt ggaacgggcc cttcatcaga tgctggttgg ggatgtatgc
301tacgctgtgg acagatgatg ctggctcaag cccttatctg tagacacttg ggaagggact
361ggagctggga gaaacaaaaa gaacaaccca aagaatacca acgcatccta cagtgcttct
421tagatagaaa agattgttgc tactctatcc atcaaatggc acaaatgggt gtaggagaag
481ggaaatcaat tggagaatgg tttggaccaa atacagttgc acaggtgtta aaaaaacttg
541ctttatttga cgaatggaat tccttggctg tttatgtttc aatggataac acagtggtca
601ttgaagatat caaaaaaatg tgccgtgtcc ttcccttgag tgctgacaca gctggtgaca
661ggcctcccga ttctttaact gcttcaaacc agagtaaggg cacctctgcc tactgctcag
721cctggaaacc cctgctgctc attgtgcccc ttcgcctggg cataaaccaa atcaatcctG
781TCTATGTTGA TGCATTCAAA GAGTGTTTTA AGATGCCACA GTCTTTAGGG GCATTAGGAG
841GAAAACCAAA TAACGCGTAT TATTTCATAG GATTCTTAGG TGACGAGCTC ATCTTCTTGG
901ACCCTCATAC AACCCAGACC TTTGTTGACA CTGAAGAGAA TGGAACGGTT AATGACCAGA
961CTTTCCATTG CCTGCAGTCC CCACAGCGAA TGAACATCCT AAACCTGGAT CCTTCAGTTG
1021CATTGGGATT TTTCTGCAAA GAAGAAAAAG ACTTTGATAA CTGGTGTAGC CTTGTTCAGA
1081AGGAAATTCT AAAGGAGAAT TTAAGGATGT TTGAATTAGT TCAGAAACAT CCATCACACT
1141GGCCTCCCTT TGTACCTCCA GCCAAGCCAG AAGTGACAAC CACTGGGGCA GAATTCATTG
1201ACTCTACTGA GCAACTGGAG GAGTTTGATC TGGAGGAAGA TTTTGAGATT CTGAGTGTGT
1261ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1321GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1381TTTATATATC TTGTGGAAAG GACGAAACGA GCTAATCGAC GCCTGCCAGA CGCGTTAAGT
1441Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)