Transcript: Human NM_181527.3

Homo sapiens N(alpha)-acetyltransferase 20, NatB catalytic subunit (NAA20), transcript variant 2, mRNA.

Source:
NCBI, updated 2018-12-04
Taxon:
Homo sapiens (human)
Gene:
NAA20 (51126)
Length:
1189
CDS:
231..731

Additional Resources:

NCBI RefSeq record:
NM_181527.3
NBCI Gene record:
NAA20 (51126)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_181527.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000035543 GTGGAGAATTAATGGGTTATA pLKO.1 343 CDS 100% 13.200 10.560 N NAA20 n/a
2 TRCN0000290708 GTGGAGAATTAATGGGTTATA pLKO_005 343 CDS 100% 13.200 10.560 N NAA20 n/a
3 TRCN0000035542 GACGCTTATGATATGAGGAAA pLKO.1 636 CDS 100% 4.950 3.465 N NAA20 n/a
4 TRCN0000290774 GACGCTTATGATATGAGGAAA pLKO_005 636 CDS 100% 4.950 3.465 N NAA20 n/a
5 TRCN0000035539 GCTGCTAAACTTATGGAGTTA pLKO.1 459 CDS 100% 4.950 3.465 N NAA20 n/a
6 TRCN0000290776 GCTGCTAAACTTATGGAGTTA pLKO_005 459 CDS 100% 4.950 3.465 N NAA20 n/a
7 TRCN0000035540 CCAGGGATACTGAGAAGAAAT pLKO.1 664 CDS 100% 13.200 7.920 N NAA20 n/a
8 TRCN0000290706 CCAGGGATACTGAGAAGAAAT pLKO_005 664 CDS 100% 13.200 7.920 N NAA20 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_181527.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08226 pDONR223 100% 91.9% 89.8% None (many diffs) n/a
2 ccsbBroad304_08226 pLX_304 0% 91.9% 89.8% V5 (many diffs) n/a
3 TRCN0000473227 TGATCAAAGACGGACTACTCTAAG pLX_317 91.8% 91.9% 89.8% V5 (many diffs) n/a
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