Construct: ORF TRCN0000473227
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006954.1_s317c1
- Derived from:
- ccsbBroadEn_08226
- DNA Barcode:
- TGATCAAAGACGGACTACTCTAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAA20 (51126)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473227
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51126 | NAA20 | N(alpha)-acetyltransferase ... | NM_016100.5 | 99.8% | 99.4% | 11T>N |
| 2 | human | 51126 | NAA20 | N(alpha)-acetyltransferase ... | NM_181527.3 | 91.9% | 89.8% | (many diffs) |
| 3 | human | 51126 | NAA20 | N(alpha)-acetyltransferase ... | NM_181528.3 | 62.1% | 57.8% | 11T>N;304_305ins146;333_334ins55 |
| 4 | mouse | 67877 | Naa20 | N(alpha)-acetyltransferase ... | NM_001141965.1 | 91% | 99.4% | (many diffs) |
| 5 | mouse | 67877 | Naa20 | N(alpha)-acetyltransferase ... | NM_026425.3 | 86.1% | 93.6% | (many diffs) |
| 6 | mouse | 67877 | Naa20 | N(alpha)-acetyltransferase ... | XM_006500089.3 | 51.4% | 53.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cacgcnacgg gcctttacct gcgacgacct gTTCCGCTTC AACAACATTA 121 ACTTGGATCC ACTTACAGAA ACTTATGGGA TTCCTTTCTA CCTACAATAC CTCGCCCACT 181 GGCCAGAGTA TTTCATTGTT GCAGAGGCAC CTGGTGGAGA ATTAATGGGT TATATTATGG 241 GTAAAGCAGA AGGCTCAGTA GCTAGGGAAG AATGGCACGG GCACGTCACA GCTCTGTCTG 301 TTGCCCCAGA ATTTCGACGC CTTGGTTTGG CTGCTAAACT TATGGAGTTA CTAGAGGAGA 361 TTTCAGAAAG AAAGGGTGGA TTTTTTGTGG ATCTCTTTGT AAGAGTATCT AACCAAGTTG 421 CAGTTAACAT GTACAAGCAG TTGGGCTACA GTGTATATAG GACGGTCATA GAGTACTATT 481 CGGCCAGCAA CGGGGAGCCT GATGAGGACG CTTATGATAT GAGGAAAGCA CTTTCCAGGG 541 ATACTGAGAA GAAATCCATC ATACCATTAC CTCATCCTGT GAGGCCTGAA GACATTGAAT 601 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 721 TTTATATATC TTGTGGAAAG GACGATGATC AAAGACGGAC TACTCTAAGA CGCGTTAAGT 781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt