Transcript: Human NM_194296.1

Homo sapiens spermatogenesis associated 24 (SPATA24), mRNA.

Source:
NCBI, updated 2019-09-24
Taxon:
Homo sapiens (human)
Gene:
SPATA24 (202051)
Length:
719
CDS:
28..645

Additional Resources:

NCBI RefSeq record:
NM_194296.1
NBCI Gene record:
SPATA24 (202051)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_194296.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000268619 ATCAACTGCGGGACGTGATTG pLKO_005 86 CDS 100% 10.800 15.120 N SPATA24 n/a
2 TRCN0000268618 AGATTGAGTCTCACATTATAA pLKO_005 413 CDS 100% 15.000 10.500 N SPATA24 n/a
3 TRCN0000268621 ATGGCAAAGAGAATGAGATTA pLKO_005 452 CDS 100% 13.200 9.240 N SPATA24 n/a
4 TRCN0000268620 ACAGATCTTCAGGAATCACAT pLKO_005 504 CDS 100% 4.950 3.465 N SPATA24 n/a
5 TRCN0000201737 GCAGCTGGAGAAAGAGAAGAT pLKO.1 306 CDS 100% 4.950 2.970 N Spata24 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_194296.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13392 pDONR223 100% 75.9% 65.2% None (many diffs) n/a
2 ccsbBroad304_13392 pLX_304 0% 75.9% 65.2% V5 (many diffs) n/a
3 TRCN0000472244 CTTCAACATATCCTGTAATCCCTT pLX_317 84.5% 75.9% 65.2% V5 (many diffs) n/a
Download CSV