Construct: ORF TRCN0000472244
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007462.1_s317c1
- Derived from:
- ccsbBroadEn_13392
- DNA Barcode:
- CTTCAACATATCCTGTAATCCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPATA24 (202051)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472244
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 202051 | SPATA24 | spermatogenesis associated 24 | XM_011543254.2 | 77% | 69.9% | (many diffs) |
| 2 | human | 202051 | SPATA24 | spermatogenesis associated 24 | NM_194296.1 | 75.9% | 65.2% | (many diffs) |
| 3 | human | 202051 | SPATA24 | spermatogenesis associated 24 | XM_005271916.4 | 39.9% | 34.6% | (many diffs) |
| 4 | human | 202051 | SPATA24 | spermatogenesis associated 24 | XM_011543252.2 | 30.6% | 25.1% | (many diffs) |
| 5 | human | 202051 | SPATA24 | spermatogenesis associated 24 | XM_011543253.2 | 30.6% | 25.1% | (many diffs) |
| 6 | mouse | 71242 | Spata24 | spermatogenesis associated 24 | NM_027733.5 | 86.8% | 82.4% | (many diffs) |
| 7 | mouse | 71242 | Spata24 | spermatogenesis associated 24 | NM_029485.2 | 69.3% | 64% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 630
- ORF length:
- 564
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gacgcccctc gggtggtcga aggcggggtc aggatctgtg tgtctcgctt 121 tagatcaact gcgggacgtg attgagTCTC AGGAGGAACT AATCCACCAG CTGAGGAACG 181 TGATGGTTCT CCAGGACGAA AATTTTGTCA GTAAAGAAGA GTTCCAGGCA GTGGAGAAGA 241 AGCTGGTGGA AGAGAAAGCT GCCCATGCCA AAACCAAGGT CCTCCTGGCC AAGGAAGAGG 301 AGAAGTTACA GTTTGCCCTC GGAGAGGTAG AGGTGCTATC CAAGCAGCTG GAGAAAGAGA 361 AGCTGGCCTT TGAAAAAGCG CTCTCCAGTG TCAAGAGCAA AGTCCTTCAG GAGTCCAGCA 421 AGAAGGACCA GCTCATCACC AAGTGCAATG GTAGGCGGGA GGCCCGTGCT CTCCACCACG 481 GTTATATAAA CGGGCTCTTT CCTAACCACC CAGTGCCTGT GTCAAAGGCA GGGTTTCACT 541 CTAACACCTG TCCCCAGCTG AGGATACAGG CAGGCCCGGG TGGGCCACAC CTTCGAAAAG 601 GCCTTAAGGG ACCAGGCAGG CCACGGAAAT GCCCAACTTT CTTGTACAAA GTGGTTGATA 661 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 721 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTTCA 781 ACATATCCTG TAATCCCTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 841 tgaaagatt