Transcript: Human NR_037583.2

Homo sapiens LEM domain containing 1 (LEMD1), transcript variant 5, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
LEMD1 (93273)
Length:
743
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_037583.2
NBCI Gene record:
LEMD1 (93273)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_037583.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000451451 GTCGCTGTTTGGTTAAGTAAT pLKO_005 448 3UTR 100% 13.200 18.480 N LEMD1 n/a
2 TRCN0000148815 CCAGAATCACATATGGGACTA pLKO.1 288 3UTR 100% 0.000 0.000 N LEMD1 n/a
3 TRCN0000149833 CCAACTTGAGAAGCTTGGATT pLKO.1 147 3UTR 100% 4.950 3.465 N LEMD1 n/a
4 TRCN0000148273 CAGAATCACATATGGGACTAT pLKO.1 289 3UTR 100% 0.000 0.000 N LEMD1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_037583.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09356 pDONR223 100% 38.1% None 1_105del;187_188ins188;461_743del n/a
2 ccsbBroad304_09356 pLX_304 0% 38.1% V5 1_105del;187_188ins188;461_743del n/a
3 TRCN0000471726 ACAACCCAAATACTCTGAGTTATC pLX_317 83.1% 38.1% V5 1_105del;187_188ins188;461_743del n/a
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