Construct: ORF TRCN0000471726
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002222.1_s317c1
- Derived from:
- ccsbBroadEn_09356
- DNA Barcode:
- ACAACCCAAATACTCTGAGTTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LEMD1 (93273)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471726
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93273 | LEMD1 | LEM domain containing 1 | NM_001199050.2 | 99.8% | 99.4% | 247G>A |
| 2 | human | 93273 | LEMD1 | LEM domain containing 1 | XM_011510162.2 | 88.9% | 88.6% | 247G>A;271_336del |
| 3 | human | 93273 | LEMD1 | LEM domain containing 1 | XM_011510160.2 | 86.4% | 86.1% | 247G>A;271_354del |
| 4 | human | 93273 | LEMD1 | LEM domain containing 1 | XM_011510161.2 | 86.4% | 86.1% | 247G>A;271_354del |
| 5 | human | 93273 | LEMD1 | LEM domain containing 1 | NM_001199051.2 | 77.1% | 76.2% | 82_83ins123;124G>A |
| 6 | human | 93273 | LEMD1 | LEM domain containing 1 | XM_011510163.2 | 66.8% | 66% | 82_83ins123;124G>A;148_231del |
| 7 | human | 93273 | LEMD1 | LEM domain containing 1 | XM_011510165.2 | 61.6% | 49.2% | (many diffs) |
| 8 | human | 93273 | LEMD1 | LEM domain containing 1 | XM_011510164.2 | 61.2% | 57.3% | (many diffs) |
| 9 | human | 93273 | LEMD1 | LEM domain containing 1 | NM_001001552.5 | 59.6% | 43% | 201_202ins142;324_325ins77 |
| 10 | human | 93273 | LEMD1 | LEM domain containing 1 | NR_037583.2 | 38.1% | 1_105del;187_188ins188;461_743del | |
| 11 | human | 93273 | LEMD1 | LEM domain containing 1 | NM_001199052.2 | 37% | 21.8% | 82_83ins265;201_202ins77 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggatgtgaag tgtctgagtg actgtaaatt gcagaaccaa cttgagaagc 121 ttggattttc acctggccca atactacctt cCACCAGAAA GTTGTATGAA AAAAAGTTAG 181 TACAGTTGTT GGTCTCACCT CCCTGTGCAC CACCTGTGAT GAATGGACCC AGAGAGCTGG 241 ATGGAGCGCA GGACAGTGAT GACAGCGAAG AGCTTAATAT CATTTTGCAA GGAAATATCA 301 TACTCTCAAC AAAAAAAAGC AAGAAACTCA AAAAATGGCC TGAGGCTTCC ACCACTAAAC 361 GCAAAGCTGT AGATACCTAT TGCTTGGATT ATAAGCCTTC CAAGGGAAGA AGGTGGGCTG 421 CAAGAGCACC AAGCACCAGA ATCACATATG GGACTATCAC CAAAGAGAGA GACTACTGCG 481 CGGAAGACCA GACTATCGAG AGCTGGAGAG AAGAAGGTTT CCCAGTGGGC TTGAAGCTTG 541 CTGTGCTTGG TATTTTCATC ATTGTGGTGT TTGTCTACCT GACTGTGGAA AATAAGTCGC 601 TGTTTGGTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACAACC CAAATACTCT GAGTTATCAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt