Transcript: Mouse NR_045514.1

Mus musculus family with sequence similarity 19, member A2 (Fam19a2), transcript variant 3, non-coding RNA.

Source:
NCBI, updated 2015-02-15
Taxon:
Mus musculus (mouse)
Gene:
Fam19a2 (268354)
Length:
4398
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_045514.1
NBCI Gene record:
Fam19a2 (268354)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NR_045514.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000197571 CAAGGAAAGCTTCTGATAATT pLKO.1 1498 3UTR 100% 15.000 10.500 N Fam19a2 n/a
2 TRCN0000182812 CGCTGCACAGATGCTGTAATA pLKO.1 1610 3UTR 100% 13.200 9.240 N Fam19a2 n/a
3 TRCN0000215875 CATCGATAATGATAATCATTG pLKO.1 2694 3UTR 100% 10.800 7.560 N Fam19a2 n/a
4 TRCN0000177424 GCTGTAATAAGAACAAGATAG pLKO.1 1622 3UTR 100% 10.800 7.560 N Fam19a2 n/a
5 TRCN0000177266 GTTCCTCTGGAAACAAAGTAA pLKO.1 1820 3UTR 100% 5.625 3.938 N Fam19a2 n/a
6 TRCN0000181738 GATGCATCCATAGTGGAACAA pLKO.1 1723 3UTR 100% 4.950 3.465 N Fam19a2 n/a
7 TRCN0000182840 CAACCATCACAAAGCTCACCA pLKO.1 1560 3UTR 100% 2.640 1.848 N Fam19a2 n/a
8 TRCN0000182318 GAGCTGTTCCTCTGGAAACAA pLKO.1 1815 3UTR 100% 5.625 3.375 N Fam19a2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_045514.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13581 pDONR223 100% 7.8% None (many diffs) n/a
2 ccsbBroad304_13581 pLX_304 0% 7.8% V5 (many diffs) n/a
3 TRCN0000477466 ATATCTCGGTCGGCCGAACTTTGC pLX_317 98.5% 7.8% V5 (many diffs) n/a
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