Construct: ORF TRCN0000477466
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002535.1_s317c1
- Derived from:
- ccsbBroadEn_13581
- DNA Barcode:
- ATATCTCGGTCGGCCGAACTTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAFA2 (338811)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477466
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 338811 | TAFA2 | TAFA chemokine like family ... | NM_178539.4 | 99.4% | 98.4% | 155G>A;200G>A |
| 2 | human | 338811 | TAFA2 | TAFA chemokine like family ... | XM_011538272.2 | 99.4% | 98.4% | 155G>A;200G>A |
| 3 | human | 338811 | TAFA2 | TAFA chemokine like family ... | XM_024448964.1 | 99.4% | 98.4% | 155G>A;200G>A |
| 4 | human | 338811 | TAFA2 | TAFA chemokine like family ... | XM_024448965.1 | 99.4% | 98.4% | 155G>A;200G>A |
| 5 | human | 338811 | TAFA2 | TAFA chemokine like family ... | XM_024448966.1 | 99.4% | 98.4% | 155G>A;200G>A |
| 6 | human | 338811 | TAFA2 | TAFA chemokine like family ... | XM_024448963.1 | 98.7% | 97.7% | 1_3delATG;158G>A;203G>A |
| 7 | human | 338811 | TAFA2 | TAFA chemokine like family ... | XM_024448962.1 | 72.4% | 71.6% | 1_147del;302G>A;347G>A |
| 8 | mouse | 268354 | Fam19a2 | family with sequence simila... | NM_001252387.1 | 88.5% | 95.4% | (many diffs) |
| 9 | mouse | 268354 | Fam19a2 | family with sequence simila... | NM_182807.3 | 85.9% | 92.5% | (many diffs) |
| 10 | mouse | 268354 | Fam19a2 | family with sequence simila... | XM_017313955.1 | 84.7% | 90.3% | (many diffs) |
| 11 | mouse | 268354 | Fam19a2 | family with sequence simila... | XM_017313952.1 | 68.2% | 73.5% | (many diffs) |
| 12 | mouse | 268354 | Fam19a2 | family with sequence simila... | XM_017313954.1 | 68.2% | 73.5% | (many diffs) |
| 13 | mouse | 268354 | Fam19a2 | family with sequence simila... | XM_017313953.1 | 67.3% | 71.7% | (many diffs) |
| 14 | mouse | 268354 | Fam19a2 | family with sequence simila... | NR_045514.1 | 7.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 462
- ORF length:
- 393
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GAGTAAGAGA TACTTACAGA AAGCAACAAA AGGAAAACTG CTAATAATAA 121 TATTTATTGT AACCTTGTGG GGGAAAGTTG TATCCAGTGC AAACCATCAT AAAGCTCACC 181 ATGTTAAAAC GGGAACTTGT GAGGTGGTGG CACTCCACAG ATACTGTAAT AAGAACAAGA 241 TAGAAGAACG GTCACAAACA GTCAAGTACT CCTGCTTCCC TGGGCAGGTG GCAGGCACCA 301 CGCGAGCTGC TCCATCATGT GTGGATGCTT CAATAGTGGA ACAGAAATGG TGGTGCCATA 361 TGCAGCCATG TCTAGAGGGA GAAGAATGTA AAGTTCTTCC GGATCGGAAA GGATGGAGCT 421 GTTCCTCTGG GAATAAAGTC AAAACAACTA GGGTAACCCA TTTGCCAACT TTCTTGTACA 481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 601 AGGACGAATA TCTCGGTCGG CCGAACTTTG CACGCGTTAA GTCgacaatc aacctctgga 661 ttacaaaatt tgtgaaagat t