Transcript: Mouse NR_045528.1

Mus musculus kinesin light chain 2 (Klc2), transcript variant 2, non-coding RNA.

Source:
NCBI, updated 2015-02-15
Taxon:
Mus musculus (mouse)
Gene:
Klc2 (16594)
Length:
3064
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_045528.1
NBCI Gene record:
Klc2 (16594)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NR_045528.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000100640 CCTCTCTCTTATCCAGTGGTA pLKO.1 2617 3UTR 100% 2.640 2.112 N Klc2 n/a
2 TRCN0000335046 CCTCTCTCTTATCCAGTGGTA pLKO_005 2617 3UTR 100% 2.640 2.112 N Klc2 n/a
3 TRCN0000100644 AGCTGGTACAAAGCCTGTAAA pLKO.1 1636 3UTR 100% 13.200 9.240 N Klc2 n/a
4 TRCN0000334972 AGCTGGTACAAAGCCTGTAAA pLKO_005 1636 3UTR 100% 13.200 9.240 N Klc2 n/a
5 TRCN0000100643 CAGCTCTCTTAACTTCCTTAA pLKO.1 2061 3UTR 100% 10.800 7.560 N Klc2 n/a
6 TRCN0000100641 CCAGCTCTCTTAACTTCCTTA pLKO.1 2060 3UTR 100% 4.950 3.465 N Klc2 n/a
7 TRCN0000335045 CCAGCTCTCTTAACTTCCTTA pLKO_005 2060 3UTR 100% 4.950 3.465 N Klc2 n/a
8 TRCN0000100642 CCTGCTATTCATGAGTCAGAT pLKO.1 743 3UTR 100% 4.950 3.465 N Klc2 n/a
9 TRCN0000335043 CCTGCTATTCATGAGTCAGAT pLKO_005 743 3UTR 100% 4.950 3.465 N Klc2 n/a
10 TRCN0000348477 AGCTGAGTCAGGACGAGATAG pLKO_005 367 3UTR 100% 10.800 6.480 N Klc2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_045528.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03974 pDONR223 100% 53% None (many diffs) n/a
2 ccsbBroad304_03974 pLX_304 0% 53% V5 (many diffs) n/a
3 TRCN0000471275 GCCATTATGTATGACAGACTGTAC pLX_317 12.8% 53% V5 (many diffs) n/a
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