Construct: ORF TRCN0000471275
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015755.1_s317c1
- Derived from:
- ccsbBroadEn_03974
- DNA Barcode:
- GCCATTATGTATGACAGACTGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLC2 (64837)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471275
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64837 | KLC2 | kinesin light chain 2 | NM_001134775.1 | 100% | 100% | |
| 2 | human | 64837 | KLC2 | kinesin light chain 2 | NM_001134776.2 | 100% | 100% | |
| 3 | human | 64837 | KLC2 | kinesin light chain 2 | NM_001318734.2 | 100% | 100% | |
| 4 | human | 64837 | KLC2 | kinesin light chain 2 | NM_022822.3 | 100% | 100% | |
| 5 | human | 64837 | KLC2 | kinesin light chain 2 | XM_005274183.1 | 100% | 100% | |
| 6 | human | 64837 | KLC2 | kinesin light chain 2 | XM_005274184.1 | 96.3% | 95.8% | (many diffs) |
| 7 | human | 64837 | KLC2 | kinesin light chain 2 | XM_024448657.1 | 96.3% | 95.8% | (many diffs) |
| 8 | human | 64837 | KLC2 | kinesin light chain 2 | NM_001134774.1 | 87.6% | 87.6% | 228_229ins231 |
| 9 | human | 64837 | KLC2 | kinesin light chain 2 | XM_017018133.1 | 64.9% | 60.8% | (many diffs) |
| 10 | mouse | 16594 | Klc2 | kinesin light chain 2 | NM_008451.2 | 87.7% | 95.4% | (many diffs) |
| 11 | mouse | 16594 | Klc2 | kinesin light chain 2 | XM_011248603.2 | 87.7% | 95.4% | (many diffs) |
| 12 | mouse | 16594 | Klc2 | kinesin light chain 2 | XM_017318069.1 | 85.4% | 92.9% | (many diffs) |
| 13 | mouse | 16594 | Klc2 | kinesin light chain 2 | XM_017318070.1 | 85.4% | 92.9% | (many diffs) |
| 14 | mouse | 16594 | Klc2 | kinesin light chain 2 | NR_045528.1 | 53% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1932
- ORF length:
- 1866
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc catgatggtg tttccgcggg aggagaagct gagccaggat gagatcgtgc 121 tgggcaccaa ggctgtcatc cagggactgg agactctgcg tggggagcat cgtgccctgc 181 tggctcctct ggttgcacct gaggccggcg aagccgagcc tggctcgcag gagcgctgca 241 tcctcctgcg tcgctccctg gaagccattg agcttgggct gggggaggcc caggtgatct 301 tggcattgtc gagccacctg ggggctgtag aatcagagaa gcagaagctg cgggcgcagg 361 tgcggcgtct ggtgcaggag aaccagtggc tgcgtgagga gctggcgggg acacagcaga 421 agctgcagcg cagtgagcag gccgtggccc agctcgagga ggagaagcag cacttgctgt 481 tcatgagcca gatccgcaag ttggatgaag acgcctcccc taacgaggag aagggggacg 541 tccccaaaga cacactggat gacctgttcc ccaatgagga tgagcagagc ccagccccta 601 gcccaggagg aggggatgtg tctggtcagc atgggggcta cgagatcccg gcccggctcc 661 gcaccctgca caacctggtg atccaatacg cctcacaggg ccgctacgag gtagctgtgc 721 cactctgcaa gcaggcactc gaagacctgg agaagacgtc aggccacgac caccctgacg 781 ttgccaccat gctgaacatc ctggcactgg tctatcggga tcagaacaag tacaaggagg 841 ctgcccacct gctcaatgat gctctggcca tccgggagaa aacactgggc aaggaccacc 901 cagccgtggc tgcgacacta aacaacctgg cagtcctgta tggcaagagg ggcaagtaca 961 aggaggctga gccattgtgc aagcgggcac tggagatccg ggagaaggtc ctgggcaagt 1021 ttcacccaga tgtggccaag cagctcagca acctggccct gctgtgccag aaccagggca 1081 aagctgagga ggtggaatat tactatcggc gggcactgga gatctatgct acacgcctcg 1141 ggcccgatga ccccaatgtg gccaagacca agaacaacct ggcttcctgc tacctgaagc 1201 agggcaagta ccaggatgcg gagaccttgt acaaggagat cctcacccgc gctcatgaga 1261 aagagtttgg ctctgtcaat ggggacaaca agcccatctg gatgcacgca gaggagcggg 1321 aggaaagcaa ggataagcgc cgggacagcg ccccctatgg ggaatacggc agctggtaca 1381 aggcctgtaa agtagacagc cccacagtca acaccaccct gcgcagcttg ggggccctat 1441 accggcgcca gggcaagctg gaagccgcgc acacactaga ggactgtgcc agccgtaacc 1501 gcaagcaggg tttggacccc gcaagccaga ccaaggtggt agaactgctg aaagatggca 1561 gtggcaggcg gggagaccgc cgcagcagcc gagacatggc tgggggtgcc gggcctcggt 1621 ctgagtctga cctcgaggac gtgggaccta cagctgagtg gaatggggat ggcagtggct 1681 ccttgaggcg cagcggttcc TTTGGGAAAC TCCGGGATGC CCTGAGGCGC AGCAGTGAGA 1741 TGCTGGTAAA GAAGCTGCAG GGGGGCACCC CCCAGGAGCC CCCTAACCCC AGGATGAAGC 1801 GGGCCAGTTC CCTCAACTTC CTCAACAAGA GCGTGGAAGA GCCGACCCAG CCTGGAGGCA 1861 CAGGTCTCTC TGACAGCCGC ACTCTCAGCT CCAGCTCCAT GGACCTCTCC CGACGAAGCT 1921 CCCTGGTGGG CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1981 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2041 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCC ATTATGTATG ACAGACTGTA 2101 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t