Construct: ORF TRCN0000471275

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015755.1_s317c1
Derived from:
ccsbBroadEn_03974
DNA Barcode:
GCCATTATGTATGACAGACTGTAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KLC2 (64837)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471275
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64837 KLC2 kinesin light chain 2 NM_001134775.1 100% 100%
2 human 64837 KLC2 kinesin light chain 2 NM_001134776.2 100% 100%
3 human 64837 KLC2 kinesin light chain 2 NM_001318734.2 100% 100%
4 human 64837 KLC2 kinesin light chain 2 NM_022822.3 100% 100%
5 human 64837 KLC2 kinesin light chain 2 XM_005274183.1 100% 100%
6 human 64837 KLC2 kinesin light chain 2 XM_005274184.1 96.3% 95.8% (many diffs)
7 human 64837 KLC2 kinesin light chain 2 XM_024448657.1 96.3% 95.8% (many diffs)
8 human 64837 KLC2 kinesin light chain 2 NM_001134774.1 87.6% 87.6% 228_229ins231
9 human 64837 KLC2 kinesin light chain 2 XM_017018133.1 64.9% 60.8% (many diffs)
10 mouse 16594 Klc2 kinesin light chain 2 NM_008451.2 87.7% 95.4% (many diffs)
11 mouse 16594 Klc2 kinesin light chain 2 XM_011248603.2 87.7% 95.4% (many diffs)
12 mouse 16594 Klc2 kinesin light chain 2 XM_017318069.1 85.4% 92.9% (many diffs)
13 mouse 16594 Klc2 kinesin light chain 2 XM_017318070.1 85.4% 92.9% (many diffs)
14 mouse 16594 Klc2 kinesin light chain 2 NR_045528.1 53% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1932
ORF length:
1866
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc catgatggtg tttccgcggg aggagaagct gagccaggat gagatcgtgc
121tgggcaccaa ggctgtcatc cagggactgg agactctgcg tggggagcat cgtgccctgc
181tggctcctct ggttgcacct gaggccggcg aagccgagcc tggctcgcag gagcgctgca
241tcctcctgcg tcgctccctg gaagccattg agcttgggct gggggaggcc caggtgatct
301tggcattgtc gagccacctg ggggctgtag aatcagagaa gcagaagctg cgggcgcagg
361tgcggcgtct ggtgcaggag aaccagtggc tgcgtgagga gctggcgggg acacagcaga
421agctgcagcg cagtgagcag gccgtggccc agctcgagga ggagaagcag cacttgctgt
481tcatgagcca gatccgcaag ttggatgaag acgcctcccc taacgaggag aagggggacg
541tccccaaaga cacactggat gacctgttcc ccaatgagga tgagcagagc ccagccccta
601gcccaggagg aggggatgtg tctggtcagc atgggggcta cgagatcccg gcccggctcc
661gcaccctgca caacctggtg atccaatacg cctcacaggg ccgctacgag gtagctgtgc
721cactctgcaa gcaggcactc gaagacctgg agaagacgtc aggccacgac caccctgacg
781ttgccaccat gctgaacatc ctggcactgg tctatcggga tcagaacaag tacaaggagg
841ctgcccacct gctcaatgat gctctggcca tccgggagaa aacactgggc aaggaccacc
901cagccgtggc tgcgacacta aacaacctgg cagtcctgta tggcaagagg ggcaagtaca
961aggaggctga gccattgtgc aagcgggcac tggagatccg ggagaaggtc ctgggcaagt
1021ttcacccaga tgtggccaag cagctcagca acctggccct gctgtgccag aaccagggca
1081aagctgagga ggtggaatat tactatcggc gggcactgga gatctatgct acacgcctcg
1141ggcccgatga ccccaatgtg gccaagacca agaacaacct ggcttcctgc tacctgaagc
1201agggcaagta ccaggatgcg gagaccttgt acaaggagat cctcacccgc gctcatgaga
1261aagagtttgg ctctgtcaat ggggacaaca agcccatctg gatgcacgca gaggagcggg
1321aggaaagcaa ggataagcgc cgggacagcg ccccctatgg ggaatacggc agctggtaca
1381aggcctgtaa agtagacagc cccacagtca acaccaccct gcgcagcttg ggggccctat
1441accggcgcca gggcaagctg gaagccgcgc acacactaga ggactgtgcc agccgtaacc
1501gcaagcaggg tttggacccc gcaagccaga ccaaggtggt agaactgctg aaagatggca
1561gtggcaggcg gggagaccgc cgcagcagcc gagacatggc tgggggtgcc gggcctcggt
1621ctgagtctga cctcgaggac gtgggaccta cagctgagtg gaatggggat ggcagtggct
1681ccttgaggcg cagcggttcc TTTGGGAAAC TCCGGGATGC CCTGAGGCGC AGCAGTGAGA
1741TGCTGGTAAA GAAGCTGCAG GGGGGCACCC CCCAGGAGCC CCCTAACCCC AGGATGAAGC
1801GGGCCAGTTC CCTCAACTTC CTCAACAAGA GCGTGGAAGA GCCGACCCAG CCTGGAGGCA
1861CAGGTCTCTC TGACAGCCGC ACTCTCAGCT CCAGCTCCAT GGACCTCTCC CGACGAAGCT
1921CCCTGGTGGG CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1981ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
2041CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCC ATTATGTATG ACAGACTGTA
2101CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)