Transcript: Human NR_045644.1

Homo sapiens SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1 (SMARCAD1), transcript variant 5, non-coding RNA.

Source:
NCBI, updated 2019-05-28
Taxon:
Homo sapiens (human)
Gene:
SMARCAD1 (56916)
Length:
5185
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_045644.1
NBCI Gene record:
SMARCAD1 (56916)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_045644.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000296009 CCTCTATCAGGAGGGTAATAA pLKO_005 1940 3UTR 100% 15.000 21.000 N SMARCAD1 n/a
2 TRCN0000296011 TCGGAATTGGAAGACCTTAAA pLKO_005 780 3UTR 100% 13.200 18.480 N SMARCAD1 n/a
3 TRCN0000050052 CCAGCACCTTATGACAATTAA pLKO.1 2249 3UTR 100% 15.000 10.500 N SMARCAD1 n/a
4 TRCN0000298882 CCAGCACCTTATGACAATTAA pLKO_005 2249 3UTR 100% 15.000 10.500 N SMARCAD1 n/a
5 TRCN0000296010 GTGATGGAGGATGGCTATAAA pLKO_005 1464 3UTR 100% 15.000 10.500 N SMARCAD1 n/a
6 TRCN0000050049 CCTTTATTACATCGCCAATAT pLKO.1 2685 3UTR 100% 13.200 9.240 N SMARCAD1 n/a
7 TRCN0000050048 GCCTTATTTGACAATGCTTAT pLKO.1 3803 3UTR 100% 10.800 7.560 N SMARCAD1 n/a
8 TRCN0000298881 GCCTTATTTGACAATGCTTAT pLKO_005 3803 3UTR 100% 10.800 7.560 N SMARCAD1 n/a
9 TRCN0000050051 GCCCTATCAGAAGGTTGGTTT pLKO.1 1820 3UTR 100% 4.950 3.465 N SMARCAD1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_045644.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08660 pDONR223 100% 59.3% None (many diffs) n/a
2 ccsbBroad304_08660 pLX_304 0% 59.3% V5 (many diffs) n/a
3 TRCN0000475171 GCACATAATGCGGGATGCGATATG pLX_317 13.6% 59.3% V5 (many diffs) n/a
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