Transcript: Human NR_146057.2

Homo sapiens potassium sodium-activated channel subfamily T member 2 (KCNT2), transcript variant 4, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
KCNT2 (343450)
Length:
5875
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_146057.2
NBCI Gene record:
KCNT2 (343450)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_146057.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000265357 ATTGGATAACCCGCCAGATAT pLKO_005 2287 3UTR 100% 13.200 18.480 N Kcnt2 n/a
2 TRCN0000056253 CCCTTAAAGATCAAGACCTAT pLKO.1 1222 3UTR 100% 4.950 6.930 N KCNT2 n/a
3 TRCN0000056255 CCTTTATGTTTCGACTGCCTT pLKO.1 2646 3UTR 100% 2.640 3.696 N KCNT2 n/a
4 TRCN0000056256 GCTAAAGGTTACCCACCTTAT pLKO.1 2024 3UTR 100% 1.080 1.512 N KCNT2 n/a
5 TRCN0000428952 CAGGTACAGGTTTCGAGATTT pLKO_005 170 3UTR 100% 13.200 10.560 N KCNT2 n/a
6 TRCN0000056254 CCAGGATTATTATGTGGTGAT pLKO.1 1112 3UTR 100% 4.050 3.240 N KCNT2 n/a
7 TRCN0000056257 CCCTTCATCTTGGAAATAATT pLKO.1 546 3UTR 100% 15.000 10.500 N KCNT2 n/a
8 TRCN0000412683 AGGTTTCAGTGGCATTGATAA pLKO_005 454 3UTR 100% 13.200 9.240 N KCNT2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_146057.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13606 pDONR223 100% 53.8% None (many diffs) n/a
2 ccsbBroad304_13606 pLX_304 0% 53.8% V5 (many diffs) n/a
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