Construct: ORF ccsbBroad304_13606
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009077.1_s304c1, BRDN0000462381
- Derived from:
- ccsbBroadEn_13606
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNT2 (343450)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13606
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 343450 | KCNT2 | potassium sodium-activated ... | NM_001287819.3 | 99.9% | 100% | 2937T>C;3333T>C |
| 2 | human | 343450 | KCNT2 | potassium sodium-activated ... | XM_006711295.3 | 99.3% | 99.3% | 2839_2859del;2958T>C;3354T>C |
| 3 | human | 343450 | KCNT2 | potassium sodium-activated ... | NM_198503.5 | 97.8% | 97.8% | 2277_2348del;3009T>C;3405T>C |
| 4 | human | 343450 | KCNT2 | potassium sodium-activated ... | XM_006711294.3 | 97.2% | 97.2% | (many diffs) |
| 5 | human | 343450 | KCNT2 | potassium sodium-activated ... | NM_001287820.3 | 94.8% | 94.9% | (many diffs) |
| 6 | human | 343450 | KCNT2 | potassium sodium-activated ... | XM_017001185.2 | 91.8% | 90.5% | (many diffs) |
| 7 | human | 343450 | KCNT2 | potassium sodium-activated ... | XM_017001179.2 | 77.4% | 74.1% | (many diffs) |
| 8 | human | 343450 | KCNT2 | potassium sodium-activated ... | XR_001737149.2 | 71.4% | (many diffs) | |
| 9 | human | 343450 | KCNT2 | potassium sodium-activated ... | XM_017001180.2 | 69.1% | 68.1% | (many diffs) |
| 10 | human | 343450 | KCNT2 | potassium sodium-activated ... | XM_011509483.3 | 68.2% | 68.3% | 2277_2277delAins1057 |
| 11 | human | 343450 | KCNT2 | potassium sodium-activated ... | XR_921774.3 | 68% | 1_251del;2528_2649del;2896_2897ins810 | |
| 12 | human | 343450 | KCNT2 | potassium sodium-activated ... | XR_001737151.2 | 64% | (many diffs) | |
| 13 | human | 343450 | KCNT2 | potassium sodium-activated ... | XM_017001181.1 | 62.9% | 62.8% | (many diffs) |
| 14 | human | 343450 | KCNT2 | potassium sodium-activated ... | XM_017001183.1 | 55.2% | 55.3% | (many diffs) |
| 15 | human | 343450 | KCNT2 | potassium sodium-activated ... | XM_024446697.1 | 55.2% | 55.3% | (many diffs) |
| 16 | human | 343450 | KCNT2 | potassium sodium-activated ... | NR_146058.2 | 54.9% | (many diffs) | |
| 17 | human | 343450 | KCNT2 | potassium sodium-activated ... | NR_146057.2 | 53.8% | (many diffs) | |
| 18 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_006529566.3 | 90.3% | 97.4% | (many diffs) |
| 19 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | NM_001081027.2 | 88.3% | 95.4% | (many diffs) |
| 20 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_006529565.3 | 87.8% | 94.8% | (many diffs) |
| 21 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_011247996.2 | 87.8% | 94.8% | (many diffs) |
| 22 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_017320509.1 | 87.3% | 94.2% | (many diffs) |
| 23 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_017320508.1 | 87.2% | 94.1% | (many diffs) |
| 24 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_006529562.3 | 85.9% | 92.8% | (many diffs) |
| 25 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_017320507.1 | 85.9% | 92.8% | (many diffs) |
| 26 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_011247998.2 | 85.8% | 92.4% | (many diffs) |
| 27 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_017320506.1 | 85.4% | 92.2% | (many diffs) |
| 28 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_006529572.3 | 84.9% | 92.1% | (many diffs) |
| 29 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_017320510.1 | 82.9% | 89.3% | (many diffs) |
| 30 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_011247997.1 | 82.2% | 88.6% | (many diffs) |
| 31 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_006529567.1 | 81.2% | 84.6% | (many diffs) |
| 32 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XR_001784492.1 | 62.7% | (many diffs) | |
| 33 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XR_001784491.1 | 62.2% | (many diffs) | |
| 34 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_011247999.2 | 60.2% | 65% | (many diffs) |
| 35 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_006529570.1 | 53.2% | 54.3% | (many diffs) |
| 36 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XM_017320512.1 | 52.7% | 56% | (many diffs) |
| 37 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XR_001784494.1 | 35.6% | (many diffs) | |
| 38 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XR_001784498.1 | 35.5% | (many diffs) | |
| 39 | mouse | 240776 | Kcnt2 | potassium channel, subfamil... | XR_001784487.1 | 33.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3402
- ORF length:
- 3333
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggttgatttg gagagcgaag tgccccctct gcctcccagg tacaggtttc 121 gagatttgct gctaggggac caaggatggc aaaacgacga cagggtacaa gttgaattct 181 atatgaatga aaatacattt aaagaaagac taaaattatt tttcataaaa aaccagagat 241 caagtctaag gatacgcctg ttcaattttt ctctcaaatt actaagctgc ttattataca 301 taatccgagt actactagaa aacccttcac aaggaaatga atggtctcat atcttttggg 361 tgaacagaag tctacctttg tggggcttac aggtttcagt ggcattgata agtctgtttg 421 aaacaatatt acttggttat cttagttata agggaaacat ctgggaacag attttacgaa 481 tacccttcat cttggaaata attaatgcag ttcccttcat tatctcaata ttctggcctt 541 ccttaaggaa tctatttgtc ccagtctttc tgaactgttg gcttgccaaa catgccttgg 601 aaaatatgat taatgatcta cacagagcca ttcagcgtac acagtctgca atgtttaatc 661 aagttttgat tttaatatct acattactat gccttatctt cacctgcatt tgtgggatcc 721 aacatctgga acgaatagga aagaagctga atctctttga ctccctttat ttctgcattg 781 tgacgttttc tactgtgggc ttcggggatg tcactcctga aacatggtcc tccaagcttt 841 ttgtagttgc tatgatttgt gttgctcttg tggttctacc catacagttt gaacagctgg 901 cttatttgtg gatggagaga caaaagtcag gaggaaacta tagtcgacat agagctcaaa 961 ctgaaaagca tgtcgtcctg tgtgtcagct cactgaagat tgatttactt atggattttt 1021 taaatgaatt ctatgctcat cctaggctcc aggattatta tgtggtgatt ttgtgtccta 1081 ctgaaatgga tgtacaggtt cgaagggtac tgcagattcc aatgtggtcc caacgagtta 1141 tctaccttca aggttcagcc cttaaagatc aagacctatt gagagcaaag atggatgacg 1201 ctgaggcctg ttttattctc agtagccgtt gtgaagtgga taggacatca tctgatcacc 1261 aaacaatttt gagagcatgg gctgtgaaag attttgctcc aaattgtcct ttgtatgtcc 1321 agatattaaa gcctgaaaat aaatttcaca tcaaatttgc tgatcatgtt gtttgtgaag 1381 aagagtttaa atacgccatg ttagctttaa actgtatatg cccagcaaca tctacactta 1441 ttacactact ggttcatacc tctagagggc aagaaggcca gcaatcgcca gaacaatggc 1501 agaagatgta cggtagatgc tccgggaatg aagtctacca cattgttttg gaagaaagta 1561 cattttttgc tgaatatgaa ggaaagagtt ttacatatgc ctctttccat gcacacaaaa 1621 agtttggcgt ctgcttgatt ggtgttagga gggaggataa taaaaacatt ttgctgaatc 1681 caggtcctcg atacattatg aattctacag acatatgctt ttatattaat attaccaaag 1741 aagagaattc agcatttaaa aaccaagacc agcagagaaa aagcaatgtg tccaggtcgt 1801 tttatcatgg accttccaga ttacctgtac atagcataat tgccagcatg ggtactgtgg 1861 ctatagactt gcaagataca agctgtagat cagcaagtgg ccctaccctg tctcttccta 1921 cagagggaag caaagaaata agaagaccta gcattgctcc tgttttagag gttgcagata 1981 catcatcgat tcaaacatgt gatcttctaa gtgaccaatc agaagatgaa actacaccag 2041 atgaagaaat gtcttcaaac ttagagtatg ctaaaggtta cccaccttat tctccatata 2101 taggaagttc acccactttt tgtcatctcc ttcatgaaaa agtaccattt tgctgcttaa 2161 gattagacaa gagttgccaa cataactact atgaggatgc aaaagcctat ggattcaaaa 2221 ataaactaat tatagttgca gctgaaacag ctggaaatgg attatataac tttattgttc 2281 ctctcagggc atattataga ccaaagaaag aacttaatcc catagtactg ctattggata 2341 accccctaga tgacttactc aggtgtggag tgacttttgc tgctaatatg gtggttgtgg 2401 ataaagagag caccatgagt gccgaggaag actacatggc agatgccaaa accattgtga 2461 acgtgcagac actcttcagg ttgttttcca gtctcagtat tatcacagag ctaactcacc 2521 ccgccaacat gagattcatg caattcagag ccaaagactg ttactctctt gctctttcaa 2581 aactggaaaa gaaagaacgg gagagaggct ctaacttggc ctttatgttt cgactgcctt 2641 ttgctgctgg gagggtgttt agcatcagta tgttggacac tctgctgtat cagtcatttg 2701 tgaaggatta tatgatttct atcacgagac ttctgttggg actggacact acaccaggat 2761 ctgggtttct ttgttctatg aaaatcactg cagatgactt atggatcaga acttatgcca 2821 gactttatca gaagttgtgt tcttctactg gagatgttcc cattggaatc tacaggactg 2881 agtctcagaa acttactaca tctgagtctc aaatatctat cagtgtagaa gagtgggaag 2941 acaccaaaga ctccaaagaa caagggcacc accgcagcaa ccaccgcaac tcaacatcca 3001 gtgaccagtc ggaccatccc ttgctgcgga gaaaaagcat gcagtgggcc cgaagactga 3061 gcagaaaagg cccaaaacac tctggtaaaa cagctgaaaa aataacccag cagcgactga 3121 acctctacag gaggtcagaa agacaagagc ttgctgaact tgtgaaaaat agaatgaaac 3181 acttgggtct ttctacagtg ggatatgatg aaatgaatga tcatcaaagt accctctcct 3241 acatcctgat taacccatct ccagatacca gaatagagct gaatgatgtt gtatacttaa 3301 ttcgaccaga tccactggcc taccttccaa acagtgagcc cagtcgaaga aacagcatct 3361 gcaatgtcac tggtcaagat tctcgggagg aaactcaact cttgccaact ttcttgtaca 3421 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 3481 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta