Transcript: Human NR_147066.1

Homo sapiens thymocyte expressed, positive selection associated 1 (TESPA1), transcript variant 17, non-coding RNA.

Source:
NCBI, updated 2019-02-06
Taxon:
Homo sapiens (human)
Gene:
TESPA1 (9840)
Length:
3716
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_147066.1
NBCI Gene record:
TESPA1 (9840)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_147066.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000268589 CCAGAACTGCGTCGGTCTTTA pLKO_005 1418 3UTR 100% 13.200 9.240 N TESPA1 n/a
2 TRCN0000268527 GCATGTGGTCTGACAGAATTG pLKO_005 1650 3UTR 100% 10.800 7.560 N TESPA1 n/a
3 TRCN0000268588 TGTTGGAAGTGATGGACAAAG pLKO_005 954 3UTR 100% 10.800 7.560 N TESPA1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_147066.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11422 pDONR223 100% 30.2% None (many diffs) n/a
2 ccsbBroadEn_11421 pDONR223 100% 29.4% None 1_464del;662_663ins43;1571_3716del n/a
3 ccsbBroad304_11421 pLX_304 0% 29.4% V5 1_464del;662_663ins43;1571_3716del n/a
4 TRCN0000467039 TTGTCCAAACTAATCCGTTCCGGA pLX_317 23.9% 29.4% V5 1_464del;662_663ins43;1571_3716del n/a
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