Construct: ORF TRCN0000467039
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014105.1_s317c1
- Derived from:
- ccsbBroadEn_11421
- DNA Barcode:
- TTGTCCAAACTAATCCGTTCCGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TESPA1 (9840)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467039
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NM_001261844.1 | 100% | 100% | |
| 2 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NM_001351148.1 | 100% | 100% | |
| 3 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NM_014796.2 | 100% | 100% | |
| 4 | human | 9840 | TESPA1 | thymocyte expressed, positi... | XM_005269247.2 | 100% | 100% | |
| 5 | human | 9840 | TESPA1 | thymocyte expressed, positi... | XM_024449286.1 | 100% | 100% | |
| 6 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NM_001351150.1 | 81.4% | 81.4% | 0_1ins213 |
| 7 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NM_001098815.3 | 73.5% | 73.5% | 1_414del |
| 8 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NM_001136030.3 | 73.5% | 73.5% | 1_414del |
| 9 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NM_001351149.2 | 73.5% | 73.5% | 1_414del |
| 10 | human | 9840 | TESPA1 | thymocyte expressed, positi... | XM_006719715.3 | 73.5% | 73.5% | 1_414del |
| 11 | human | 9840 | TESPA1 | thymocyte expressed, positi... | XM_011539035.2 | 73.5% | 73.5% | 1_414del |
| 12 | human | 9840 | TESPA1 | thymocyte expressed, positi... | XM_011539037.3 | 73.5% | 73.5% | 1_414del |
| 13 | human | 9840 | TESPA1 | thymocyte expressed, positi... | XM_017020262.1 | 73.5% | 73.5% | 1_414del |
| 14 | human | 9840 | TESPA1 | thymocyte expressed, positi... | XM_017020263.1 | 73.5% | 73.5% | 1_414del |
| 15 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NM_001351151.1 | 69.9% | 69.9% | 0_1ins345 |
| 16 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NM_001351152.1 | 69.9% | 69.9% | 0_1ins345 |
| 17 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NM_001351153.1 | 69.9% | 69.9% | 0_1ins345 |
| 18 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NM_001351154.1 | 69.9% | 69.9% | 0_1ins345 |
| 19 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NM_001351155.1 | 69.9% | 69.9% | 0_1ins345 |
| 20 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NR_147065.1 | 31.3% | 1_373del;1523_3668del | |
| 21 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NR_147068.1 | 31.2% | (many diffs) | |
| 22 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NR_147067.1 | 30.5% | 1_464del;1614_3759del | |
| 23 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NR_147070.1 | 30.4% | 1_464del;705_706insGCAG;1610_3755del | |
| 24 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NR_147069.1 | 30% | (many diffs) | |
| 25 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NR_147073.1 | 29.8% | 1_552del;1702_3847del | |
| 26 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NR_147066.1 | 29.4% | 1_464del;662_663ins43;1571_3716del | |
| 27 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NR_147071.1 | 29.3% | 1_464del;659_660ins47;1567_3712del | |
| 28 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NR_147064.1 | 29.3% | 1_626del;1776_3921del | |
| 29 | human | 9840 | TESPA1 | thymocyte expressed, positi... | XR_001748931.1 | 28.7% | 1_413del;1563_3992del | |
| 30 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NR_147072.1 | 28.5% | 1_585del;783_784ins43;1692_3837del | |
| 31 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NR_147063.2 | 27.2% | 1_637del;1787_4220del | |
| 32 | human | 9840 | TESPA1 | thymocyte expressed, positi... | NR_147062.2 | 26.1% | 1_810del;1960_4393del | |
| 33 | human | 9840 | TESPA1 | thymocyte expressed, positi... | XR_001748928.1 | 20.5% | 1_2018del;3168_5597del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1218
- ORF length:
- 1149
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gactgggggg accaacaaga ctagttcaag catctcagaa attctggaca 121 aagtgcaaga agatgcagaa gatgtcctct tcagtctggg ctttggccaa gaggatcaca 181 aagacacttc tcggataccc gcccgatttt tcaccacccc ctctcaggcc aagggcattg 241 atttccagct cttcctgaag tcccaggtgc ggaggattga aatggaagac ccctgcctca 301 tgctggccag caggtttaag caggtgcaaa cactggctgt cactgctgat gccttcttct 361 gtctctactc ctatgtgtct aagacacctg tccaaaagtt cacaccatcc cacatgttct 421 ggaattgcaa ccatccaact gatgtgccat ccatcaggat tctgtcccga gagcctgaac 481 cccagtcacc cagagaccgc cttcggaaag ccatctccaa gatgtgtctg tacacatgcc 541 cccgagaccg gccaccacca ccccacaaca cccccaaaag gaacagtttg gaccaagttg 601 tgttggaagt gatggacaaa gtgaaagaag agaagcagtt cctccagcaa gactctgatt 661 tggggcaatt ctcacaggaa gatcctgtgc cccctgctga gggtaagaag ttgcccactt 721 ctccctatcc atgtgtcttc tgctgtgaag aggaaacaca gcagaggatg tccacagtgc 781 tagcaccatc ccaaactctg gattcgaacc ccaaggtacc ctgttgcaca cattctctgc 841 ccatagaaga tccacagtgg agcacagacc cagctcagat aaggagagag ctgtgtagtc 901 taccagccac caatacggaa acccatccag ccaaggatga gactttcTGG AAAAGAAAGA 961 GCAGAGCAAG AAAGAGCCTC TTCCAGAAGA ATCTCATGGG CAGGAAGGTT AAGTCCTTGG 1021 ACCTGTCCAT CACCCAGCAG AAATGGAAGC AGAGTGTAGA CAGACCAGAA CTGCGTCGGT 1081 CTTTAAGCCA GCAGCCACAG GACACTTTTG ACTTGGAGGA GGTGCAAAGC AACAGTGAGG 1141 AGGAGCAGAG TCAGAGTCGC TGGCCCAGCA GACCCAGGCA CCCCCACCAC CACCAGACTT 1201 TTGCTGGCAA AGACTCGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1261 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1321 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTGTCCA AACTAATCCG 1381 TTCCGGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt