Transcript: Mouse NM_177561.3

Mus musculus ubiquitin specific peptidase 46 (Usp46), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Usp46 (69727)
Length:
3225
CDS:
127..1227

Additional Resources:

NCBI RefSeq record:
NM_177561.3
NBCI Gene record:
Usp46 (69727)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_177561.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000087216 GCATTACATCACCATCGTAAA pLKO.1 1062 CDS 100% 10.800 15.120 N Usp46 n/a
2 TRCN0000022402 GCTAAACACTATTGCGGACAT pLKO.1 510 CDS 100% 4.050 5.670 N USP46 n/a
3 TRCN0000315060 GCTAAACACTATTGCGGACAT pLKO_005 510 CDS 100% 4.050 5.670 N USP46 n/a
4 TRCN0000087213 CCCTCCATGAAGCATATTTAT pLKO.1 3002 3UTR 100% 15.000 10.500 N Usp46 n/a
5 TRCN0000087215 GCTCACGAGTTCTTAAATTAT pLKO.1 487 CDS 100% 15.000 10.500 N Usp46 n/a
6 TRCN0000087217 CCAGAGCAGTTTCCGATCAAT pLKO.1 199 CDS 100% 5.625 3.938 N Usp46 n/a
7 TRCN0000087214 GCCGAGAACAATAAGCCAGAA pLKO.1 598 CDS 100% 4.050 2.835 N Usp46 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_177561.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08876 pDONR223 100% 93.1% 100% None (many diffs) n/a
2 ccsbBroad304_08876 pLX_304 0% 93.1% 100% V5 (many diffs) n/a
3 TRCN0000481093 AAGTTCAGCCCTGAACCCGCATGT pLX_317 43.7% 93.1% 100% V5 (many diffs) n/a
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