Transcript: Human XM_017026387.1

PREDICTED: Homo sapiens cytochrome P450 family 2 subfamily F member 1 (CYP2F1), transcript variant X11, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CYP2F1 (1572)
Length:
1228
CDS:
179..1123

Additional Resources:

NCBI RefSeq record:
XM_017026387.1
NBCI Gene record:
CYP2F1 (1572)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017026387.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000064192 CCTGCTGATGACCACACATAA pLKO.1 1103 CDS 100% 13.200 9.240 N CYP2F1 n/a
2 TRCN0000420696 GACCCTAAGCTCAAGAGATAA pLKO_005 463 CDS 100% 13.200 9.240 N CYP2F1 n/a
3 TRCN0000413697 ATGCTGACTTCTCTCACTAAG pLKO_005 554 CDS 100% 10.800 7.560 N CYP2F1 n/a
4 TRCN0000064191 CTATCCAGATTCTACGGAATT pLKO.1 786 CDS 100% 0.000 0.000 N CYP2F1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017026387.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13840 pDONR223 100% 60.4% 43.3% None (many diffs) n/a
2 ccsbBroad304_13840 pLX_304 0% 60.4% 43.3% V5 (many diffs) n/a
3 TRCN0000475507 GTCGAAACTTACACGTGGCACTCC pLX_317 51.8% 60.4% 43.3% V5 (many diffs) n/a
Download CSV