Transcript: Human XM_011531178.2

PREDICTED: Homo sapiens NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NSDHL (50814)
Length:
1690
CDS:
322..1443

Additional Resources:

NCBI RefSeq record:
XM_011531178.2
NBCI Gene record:
NSDHL (50814)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011531178.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000335872 CCAAGTCGCACGGACTCATTT pLKO_005 354 CDS 100% 13.200 18.480 N NSDHL n/a
2 TRCN0000335869 TCCTGACAGGCCTCAATTATG pLKO_005 1166 CDS 100% 13.200 18.480 N NSDHL n/a
3 TRCN0000026431 GCGTCGATATCAAGAATGGAA pLKO.1 776 CDS 100% 3.000 4.200 N NSDHL n/a
4 TRCN0000335870 CCCTGTGGATTGATGAAATAA pLKO_005 1497 3UTR 100% 15.000 10.500 N NSDHL n/a
5 TRCN0000335871 AGGATATGCTGTCAATGTATT pLKO_005 501 CDS 100% 13.200 9.240 N NSDHL n/a
6 TRCN0000026483 GCACCAAGAATGTCATTGAAA pLKO.1 692 CDS 100% 5.625 3.938 N NSDHL n/a
7 TRCN0000026475 CCCACCATCCAGTAACAACAA pLKO.1 642 CDS 100% 4.950 3.465 N NSDHL n/a
8 TRCN0000026496 CGGCAAGATGAAGTTCGTGAT pLKO.1 984 CDS 100% 4.050 2.835 N NSDHL n/a
9 TRCN0000335951 CGGCAAGATGAAGTTCGTGAT pLKO_005 984 CDS 100% 4.050 2.835 N NSDHL n/a
10 TRCN0000026461 GCTGGGCTCTCTCGACACGTT pLKO.1 1456 3UTR 100% 0.000 0.000 N NSDHL n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011531178.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08183 pDONR223 100% 99.9% 100% None 132T>G n/a
2 ccsbBroad304_08183 pLX_304 0% 99.9% 100% V5 132T>G n/a
3 TRCN0000471342 ATTCTCAGAACAATTGCCATCCCA pLX_317 42.7% 99.9% 100% V5 132T>G n/a
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