Transcript: Human XM_017012440.2

PREDICTED: Homo sapiens kelch like family member 7 (KLHL7), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KLHL7 (55975)
Length:
1682
CDS:
224..1201

Additional Resources:

NCBI RefSeq record:
XM_017012440.2
NBCI Gene record:
KLHL7 (55975)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017012440.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000249379 TACAAGCTGAACCACTTATTC pLKO_005 963 CDS 100% 13.200 10.560 N Klhl7 n/a
2 TRCN0000249383 AGATGCTGAACCTGATATTAT pLKO_005 487 CDS 100% 15.000 10.500 N Klhl7 n/a
3 TRCN0000134868 CTGCTAGAATTTCCGTGAATA pLKO.1 534 CDS 100% 13.200 9.240 N KLHL7 n/a
4 TRCN0000275531 CTGCTAGAATTTCCGTGAATA pLKO_005 534 CDS 100% 13.200 9.240 N KLHL7 n/a
5 TRCN0000136619 GCGAGTAACACATCTTCTCAA pLKO.1 787 CDS 100% 4.950 3.465 N KLHL7 n/a
6 TRCN0000275532 GCGAGTAACACATCTTCTCAA pLKO_005 787 CDS 100% 4.950 3.465 N KLHL7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017012440.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15920 pDONR223 0% 41% 34.6% None (many diffs) n/a
2 ccsbBroad304_15920 pLX_304 0% 41% 34.6% V5 (many diffs) n/a
3 TRCN0000477622 CTAGAGAGATTTAGCCCGCTCTCA pLX_317 92% 41% 34.6% V5 (many diffs) n/a
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