Transcript: Human XM_005244743.3

PREDICTED: Homo sapiens chromosome 1 open reading frame 174 (C1orf174), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
C1orf174 (339448)
Length:
1962
CDS:
371..1135

Additional Resources:

NCBI RefSeq record:
XM_005244743.3
NBCI Gene record:
C1orf174 (339448)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005244743.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000130329 GCTTTGGTCATAGGCAAGTTT pLKO.1 1793 3UTR 100% 5.625 7.875 N C1orf174 n/a
2 TRCN0000352329 GCTTTGGTCATAGGCAAGTTT pLKO_005 1793 3UTR 100% 5.625 7.875 N C1orf174 n/a
3 TRCN0000130967 CGCTTGAAAGCACGAAGTTGT pLKO.1 150 5UTR 100% 4.950 6.930 N C1orf174 n/a
4 TRCN0000128813 CGAACGTCCAAGAAGTTCAAA pLKO.1 563 CDS 100% 5.625 4.500 N C1orf174 n/a
5 TRCN0000352422 GAACGTCCAAGAAGTTCAAAT pLKO_005 564 CDS 100% 13.200 9.240 N C1orf174 n/a
6 TRCN0000130157 CGACGATGATGCAGAAATGTA pLKO.1 1114 CDS 100% 5.625 3.938 N C1orf174 n/a
7 TRCN0000352402 CGACGATGATGCAGAAATGTA pLKO_005 1114 CDS 100% 5.625 3.938 N C1orf174 n/a
8 TRCN0000129532 GAGTGACTCTCGCTTAGCAAA pLKO.1 784 CDS 100% 4.950 3.465 N C1orf174 n/a
9 TRCN0000352435 GAGTGACTCTCGCTTAGCAAA pLKO_005 784 CDS 100% 4.950 3.465 N C1orf174 n/a
10 TRCN0000129021 GCTTGAAAGCACGAAGTTGTT pLKO.1 151 5UTR 100% 4.950 3.465 N C1orf174 n/a
11 TRCN0000129405 GATGATGACGACGACGATGAT pLKO.1 1103 CDS 100% 4.950 2.475 Y C1orf174 n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1639 3UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1639 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005244743.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10015 pDONR223 100% 86.9% 77.6% None (many diffs) n/a
2 ccsbBroad304_10015 pLX_304 0% 86.9% 77.6% V5 (many diffs) n/a
3 TRCN0000480625 AGGGCAAAACTGGCAGCGGACACG pLX_317 51.6% 86.9% 77.6% V5 (many diffs) n/a
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