Transcript: Human XM_005263140.3

PREDICTED: Homo sapiens 3-hydroxybutyrate dehydrogenase 2 (BDH2), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
BDH2 (56898)
Length:
2332
CDS:
101..724

Additional Resources:

NCBI RefSeq record:
XM_005263140.3
NBCI Gene record:
BDH2 (56898)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005263140.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000244983 ACGGATGCCATCCCTTATTAT pLKO_005 1881 3UTR 100% 15.000 21.000 N BDH2 n/a
2 TRCN0000244982 TAGCCATGCTCTGCGTGTATT pLKO_005 636 CDS 100% 13.200 18.480 N BDH2 n/a
3 TRCN0000244981 ACTGGGACTTCTCGATGAATC pLKO_005 390 CDS 100% 10.800 15.120 N BDH2 n/a
4 TRCN0000036736 GACTGGGACTTCTCGATGAAT pLKO.1 389 CDS 100% 5.625 7.875 N BDH2 n/a
5 TRCN0000046506 CCTTGATGTCACAAAGAAGAA pLKO.1 268 CDS 100% 4.950 3.960 N BDH2P1 n/a
6 TRCN0000036735 GCTCAGAAATCTGGCAATATT pLKO.1 464 CDS 100% 15.000 10.500 N BDH2 n/a
7 TRCN0000244979 TCATAGCCACAGACATTAATG pLKO_005 198 CDS 100% 13.200 9.240 N BDH2 n/a
8 TRCN0000046504 GCTTCTGATGAATCTGCTTAT pLKO.1 659 CDS 100% 10.800 7.560 N BDH2P1 n/a
9 TRCN0000036738 CGCCATCTCTACAAGAAAGAA pLKO.1 531 CDS 100% 5.625 3.938 N BDH2 n/a
10 TRCN0000046505 CCAAACTTCAGGAACTGGAAA pLKO.1 222 CDS 100% 4.950 3.465 N BDH2P1 n/a
11 TRCN0000046503 GCCAATGAAGTTGAGAGACTT pLKO.1 305 CDS 100% 4.950 3.465 N BDH2P1 n/a
12 TRCN0000036737 CCAAAGTCATAGCCACAGACA pLKO.1 192 CDS 100% 2.640 1.848 N BDH2 n/a
13 TRCN0000244980 AGACTTGATGTTCTCTTTAAT pLKO_005 320 CDS 100% 15.000 9.000 N BDH2 n/a
14 TRCN0000046507 CTGTCATCATTGATGGAGGAT pLKO.1 693 CDS 100% 2.640 3.696 N BDH2P1 n/a
15 TRCN0000041859 CCAAAGTCATAGCCACAGATA pLKO.1 192 CDS 100% 4.950 3.465 N Bdh2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005263140.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08656 pDONR223 100% 84.3% 84% None 209A>G;416_417ins114 n/a
2 ccsbBroad304_08656 pLX_304 0% 84.3% 84% V5 209A>G;416_417ins114 n/a
3 TRCN0000469483 CCAGCGGAACGGAGGATGATAAAC pLX_317 52% 84.3% 84% V5 209A>G;416_417ins114 n/a
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