Transcript: Human XM_005268543.5

PREDICTED: Homo sapiens ring finger protein 14 (RNF14), transcript variant X12, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RNF14 (9604)
Length:
2401
CDS:
112..1008

Additional Resources:

NCBI RefSeq record:
XM_005268543.5
NBCI Gene record:
RNF14 (9604)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005268543.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231558 GTACTGGCTGTATGCAATATT pLKO_005 848 CDS 100% 15.000 21.000 N RNF14 n/a
2 TRCN0000231559 TGGATGTTGACGACGATATTT pLKO_005 965 CDS 100% 15.000 21.000 N RNF14 n/a
3 TRCN0000231557 TTAGTGGAAGCAGAGTTATTT pLKO_005 427 CDS 100% 15.000 21.000 N RNF14 n/a
4 TRCN0000003444 CCCAAAGCTCTGAATAGTTAA pLKO.1 1265 3UTR 100% 13.200 18.480 N RNF14 n/a
5 TRCN0000257357 CAAGCGGATGAGGCTAATAAA pLKO_005 679 CDS 100% 15.000 12.000 N RNF14 n/a
6 TRCN0000231560 AGTAACTTTGCGGGATATTTA pLKO_005 1071 3UTR 100% 15.000 10.500 N RNF14 n/a
7 TRCN0000003442 AGGTATGGTAAGAGAGTGATT pLKO.1 715 CDS 100% 4.950 3.465 N RNF14 n/a
8 TRCN0000003443 CCACCTTCATTCACACTTAGT pLKO.1 373 CDS 100% 4.950 3.465 N RNF14 n/a
9 TRCN0000010786 CTGTGGATGTTGACGACGATA pLKO.1 962 CDS 100% 4.950 3.465 N RNF14 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005268543.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02209 pDONR223 100% 62.8% 62.8% None 302_303ins528 n/a
2 ccsbBroad304_02209 pLX_304 0% 62.8% 62.8% V5 302_303ins528 n/a
3 TRCN0000469427 TGAAGGTAACCAAAGAAGTGTCGC pLX_317 27.4% 62.8% 62.8% V5 302_303ins528 n/a
Download CSV