Transcript: Human XM_005268676.3

PREDICTED: Homo sapiens keratin 80 (KRT80), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KRT80 (144501)
Length:
3954
CDS:
89..1552

Additional Resources:

NCBI RefSeq record:
XM_005268676.3
NBCI Gene record:
KRT80 (144501)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005268676.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000416750 CTGATGGGTGAGTCGGAATTA pLKO_005 1810 3UTR 100% 13.200 18.480 N KRT80 n/a
2 TRCN0000116585 CTACTCTCCTTTATGCCCAAT pLKO.1 539 CDS 100% 4.050 5.670 N KRT80 n/a
3 TRCN0000416243 GGGCATCTCTATGAGGAATAT pLKO_005 284 CDS 100% 13.200 9.240 N KRT80 n/a
4 TRCN0000425392 CTTAAGCTAGACTCAAGAAAG pLKO_005 1602 3UTR 100% 10.800 7.560 N KRT80 n/a
5 TRCN0000116584 CTCTCCTTTATGCCCAATCAA pLKO.1 542 CDS 100% 5.625 3.938 N KRT80 n/a
6 TRCN0000116583 GACATGGAGTTCACCTTTGTT pLKO.1 437 CDS 100% 5.625 3.938 N KRT80 n/a
7 TRCN0000116582 CCCTTCCTGTTTCTGCATGAT pLKO.1 3278 3UTR 100% 4.950 3.465 N KRT80 n/a
8 TRCN0000116586 CGAAATGTCAGAGAAGTACTT pLKO.1 1504 CDS 100% 4.950 3.465 N KRT80 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005268676.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09612 pDONR223 100% 61.9% 59.2% None (many diffs) n/a
2 ccsbBroad304_09612 pLX_304 0% 61.9% 59.2% V5 (many diffs) n/a
3 TRCN0000491718 ACGGCCATTACGTGCGTGGGATGC pLX_317 31.3% 61.9% 59.2% V5 (many diffs) n/a
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