Construct: ORF TRCN0000491718
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005180.1_s317c1
- Derived from:
- ccsbBroadEn_09612
- DNA Barcode:
- ACGGCCATTACGTGCGTGGGATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT80 (144501)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491718
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144501 | KRT80 | keratin 80 | NM_001081492.2 | 99.8% | 100% | 1113C>T;1254C>A |
| 2 | human | 144501 | KRT80 | keratin 80 | NM_182507.3 | 91.9% | 90.7% | (many diffs) |
| 3 | human | 144501 | KRT80 | keratin 80 | XM_005268676.3 | 61.9% | 59.2% | (many diffs) |
| 4 | mouse | 74127 | Krt80 | keratin 80 | NM_028770.2 | 79.2% | 78.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1332
- ORF length:
- 1266
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctgccgctcc tgcgtggttg gcttcagcag cctcagcagc tgtgaggtga 121 ccccggtggg cagcccccgg cctggaacct caggatggga cagctgcagg gcccccgggc 181 cgggcttcag ctcccgcagc ctcacaggct gctggtcggc tggcactatc tccaaggtga 241 ctgtgaaccc cggcctgctg gtgcccctgg atgtcaagtt ggaccccgct gttcagcagc 301 tgaagaacca ggagaaggag gagatgaagg ccctcaatga taaatttgcc tccctaattg 361 gcaaggtgca agccctggaa cagcgcaacc agctgctgga gacacgctgg agcttcctgc 421 agggccagga ctcagccatc ttcgacctcg ggcatctcta tgaggaatat cagggccggc 481 tgcaggagga actgcgcaaa gtgagccagg agcgggggca gctggaggcc aacctgctgc 541 aggtgctgga gaaggttgag gagtttcgaa tcaggtatga ggatgagatc tccaagcgca 601 cagacatgga gttcaccttt gttcagctga agaaggacct ggatgcagag tgtcttcatc 661 ggactgaact ggaaaccaag ttaaaaagcc tggagagctt cgtggagttg atgaaaacca 721 tctatgagca ggagctgaag gacctggcag cacaggtgaa ggatgtgtcg gtgaccgtcg 781 gcatggacag ccgctgccac atcgacctga gcggcatcgt ggaggaggtg aaggcccagt 841 atgacgccgt cgcggctcgc agcctggagg aggccgaggc atactctcgg agccagctgg 901 aggagcaggc cgcccgctcg gccgagtatg ggagcagccT CCAGAGCAGC CGCAGCGAGA 961 TCGCGGATCT CAATGTGCGC ATCCAGAAGC TGCGGTCCCA GATCCTCTCT GTCAAGAGCC 1021 ATTGCCTGAA ACTGGAGGAG AACATCAAGA CAGCTGAGGA GCAGGGTGAG CTGGCCTTCC 1081 AGGATGCCAA GACCAAGCTG GCCCAGCTGG AGGCCGCCCT GCAGCAGGCC AAGCAGGACA 1141 TGGCGCGGCA GCTGCGCAAG TACCAGGAGC TGATGAATGT CAAGCTGGCC CTGGACATCG 1201 AGATCGCCAC CTACAGGAAG CTGGTGGAGG GCGAGGAGGG CAGGATGGAC TCGCCCTCAG 1261 CCACTGTGGT CAGCGCTGTG CAGTCCAGGT GCAAAACCGC CCCTTCCCTC CCCTACCCAT 1321 TATGTTCCCT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1381 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1441 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACG GCCATTACGT GCGTGGGATG 1501 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t