Transcript: Human XM_005269247.2

PREDICTED: Homo sapiens thymocyte expressed, positive selection associated 1 (TESPA1), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TESPA1 (9840)
Length:
3972
CDS:
448..1599

Additional Resources:

NCBI RefSeq record:
XM_005269247.2
NBCI Gene record:
TESPA1 (9840)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005269247.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000283738 CCAGGTGCGGAGGATTGAAAT pLKO_005 642 CDS 100% 13.200 18.480 N TESPA1 n/a
2 TRCN0000268528 GAAGGGAATCCAATCAATAAA pLKO_005 72 5UTR 100% 15.000 10.500 N TESPA1 n/a
3 TRCN0000268589 CCAGAACTGCGTCGGTCTTTA pLKO_005 1444 CDS 100% 13.200 9.240 N TESPA1 n/a
4 TRCN0000268527 GCATGTGGTCTGACAGAATTG pLKO_005 1622 3UTR 100% 10.800 7.560 N TESPA1 n/a
5 TRCN0000268588 TGTTGGAAGTGATGGACAAAG pLKO_005 980 CDS 100% 10.800 7.560 N TESPA1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005269247.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11421 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_11421 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000467039 TTGTCCAAACTAATCCGTTCCGGA pLX_317 23.9% 100% 100% V5 n/a
4 ccsbBroadEn_11422 pDONR223 100% 96.7% 96.4% None (many diffs) n/a
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