Transcript: Mouse XM_006506582.3

PREDICTED: Mus musculus tRNA nucleotidyl transferase, CCA-adding, 1 (Trnt1), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Trnt1 (70047)
Length:
2437
CDS:
536..1576

Additional Resources:

NCBI RefSeq record:
XM_006506582.3
NBCI Gene record:
Trnt1 (70047)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006506582.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000111516 CGAATTGATGTTACCACTGAT pLKO.1 647 CDS 100% 4.950 3.960 N Trnt1 n/a
2 TRCN0000306527 ACGAAGAGATCTCACTATAAA pLKO_005 718 CDS 100% 15.000 10.500 N Trnt1 n/a
3 TRCN0000306528 TCAATGGTTATGCAGATTTAA pLKO_005 780 CDS 100% 15.000 10.500 N Trnt1 n/a
4 TRCN0000306526 ACAGGAGGTTGGCAGATATTC pLKO_005 1947 3UTR 100% 13.200 9.240 N Trnt1 n/a
5 TRCN0000377071 ATTTGATCCACCTGATCTATG pLKO_005 1029 CDS 100% 10.800 7.560 N Trnt1 n/a
6 TRCN0000111519 CACATAGGTTTACCTGCTAAT pLKO.1 1067 CDS 100% 10.800 7.560 N Trnt1 n/a
7 TRCN0000111515 GCCCTGTGTTTATCTCCTTTA pLKO.1 2305 3UTR 100% 10.800 7.560 N Trnt1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006506582.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11949 pDONR223 100% 75.4% 80.4% None (many diffs) n/a
2 ccsbBroad304_11949 pLX_304 0% 75.4% 80.4% V5 (many diffs) n/a
3 TRCN0000473813 CGTTCAGAAAACATGCATAGCTCT pLX_317 40.9% 75.4% 80.4% V5 (many diffs) n/a
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