Construct: ORF TRCN0000473813
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009853.1_s317c1
- Derived from:
- ccsbBroadEn_11949
- DNA Barcode:
- CGTTCAGAAAACATGCATAGCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRNT1 (51095)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473813
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NM_001367321.1 | 93.2% | 93.3% | 1_87del;948A>G |
| 2 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NM_001367322.1 | 93.2% | 93.3% | 1_87del;948A>G |
| 3 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NM_001367323.1 | 93.2% | 93.3% | 1_87del;948A>G |
| 4 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NM_182916.3 | 93.2% | 93.3% | 1_87del;948A>G |
| 5 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NM_001302946.2 | 88.6% | 88.7% | 1_87del;741_742ins60;888A>G |
| 6 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159939.1 | 47.7% | (many diffs) | |
| 7 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159940.1 | 47.4% | (many diffs) | |
| 8 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | XR_001740168.2 | 47.2% | 1_168del;1029A>G;1384_2567del | |
| 9 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159935.1 | 47% | 1_165del;1026A>G;1381_2578del | |
| 10 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159938.1 | 45.2% | (many diffs) | |
| 11 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159941.1 | 36.8% | (many diffs) | |
| 12 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | XR_001740169.2 | 34.8% | 1_168del;1029A>G;1384_3487del | |
| 13 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159934.1 | 27.2% | 1_165del;1026A>G;1381_4452del | |
| 14 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159936.1 | 22.9% | (many diffs) | |
| 15 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159937.1 | 22% | (many diffs) | |
| 16 | mouse | 70047 | Trnt1 | tRNA nucleotidyl transferas... | NM_001242358.1 | 82.6% | 88.2% | (many diffs) |
| 17 | mouse | 70047 | Trnt1 | tRNA nucleotidyl transferas... | NM_027296.3 | 82.6% | 88.2% | (many diffs) |
| 18 | mouse | 70047 | Trnt1 | tRNA nucleotidyl transferas... | XM_006506581.3 | 82.6% | 88.2% | (many diffs) |
| 19 | mouse | 70047 | Trnt1 | tRNA nucleotidyl transferas... | XM_006506582.3 | 75.4% | 80.4% | (many diffs) |
| 20 | mouse | 70047 | Trnt1 | tRNA nucleotidyl transferas... | NM_001242360.1 | 37.1% | 39.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1281
- ORF length:
- 1215
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gttgcagtct cccgaattcc agtcactttt cacagaagga ctgaagagtc 121 tgacagaatt atttgtcaaa gagaatcacg aattaagaat agcaggagga gcagtgaggg 181 atttattaaa tggagtaaag cctcaggata tagattttgc caccactgct acccctactc 241 aaatgaagga gatgtttcag tcggctggga ttcggatgat aaacaacaga ggagaaaagc 301 acggaacaat tactgccagg cttcatgaag aaaattttga gattactaca ctacggattg 361 atgtcaccac tgatggaaga catgctgagg tagaatttac aactgactgg cagaaagatg 421 cggaacgcag agatctcact ataaattcta tgtttttagg ttttgatggc actttatttg 481 actactttaa tggttatgaa gatttaaaaa ataagaaagt tagatttgtt ggacatgcta 541 aacagagaat acaagaggat tatcttagaa ttttaagata cttcaggttt tatgggagaa 601 ttgtagacaa acctggtgac catgatcctg agactttgga agcaattgca gaaaatgcaa 661 aaggcttggc tggaatatca ggagaaagga tttgggtgga actgaaaaaa attcttgttg 721 gtaaccatgt aaatcatttg attcacctta tctatgatct tgatgtggct ccttatatag 781 gtttaCCTGC TAATGCAAGT TTAGAAGAAT TTGACAAAGT CAGTAAAAAT GTTGATGGTT 841 TTTCACCAAA GCCAGTGACT CTTTTGGCCT CATTATTCAA AGTACAAGAT GATGTCACAA 901 AATTGGATTT GAGGTTGAAG ATCGCGAAAG AGGAGAAAAA CCTTGGCTTA TTTATAGTTA 961 AAAATAGGAA AGATTTAATT AAAGCAACAG ATAGTTCAGA CCCATTGAAA CCCTATCAAG 1021 ACTTCATTAT AGATTCTAGG GAACCTGATG CAACTACTCG TGTATGTGAA CTACTGAAGT 1081 ACCAAGGAGA GCACTGTCTC CTAAAGGAAA TGCAGCAGTG GTCCATTCCT CCATTTCCTG 1141 TAAGTGGCCA TGACATCAGA AAAGTGGGCA TTTCTTCAGG AAAAGAAATT GGGGCTCTAT 1201 TACAACAGTT GCGAGAACAG TGGAAAAAAA GTGGTTACCA AATGGAAAAA GATGAACTTC 1261 TGAGTTACAT AAAGAAGACC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1321 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1381 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGTT CAGAAAACAT 1441 GCATAGCTCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt