Transcript: Mouse XM_006507641.3

PREDICTED: Mus musculus post-GPI attachment to proteins 2 (Pgap2), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pgap2 (233575)
Length:
2049
CDS:
234..1016

Additional Resources:

NCBI RefSeq record:
XM_006507641.3
NBCI Gene record:
Pgap2 (233575)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006507641.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000262951 ACTTCCGGCACAACATGTATT pLKO_005 853 CDS 100% 13.200 10.560 N Pgap2 n/a
2 TRCN0000215409 GCATTAACTTCAGTCTCAATG pLKO.1 592 CDS 100% 10.800 8.640 N Pgap2 n/a
3 TRCN0000281571 GCATTAACTTCAGTCTCAATG pLKO_005 592 CDS 100% 10.800 8.640 N Pgap2 n/a
4 TRCN0000262952 GGAACAAAGAGCTGCTAATAA pLKO_005 967 CDS 100% 15.000 10.500 N Pgap2 n/a
5 TRCN0000173815 CGGGAACAAAGAGCTGCTAAT pLKO.1 965 CDS 100% 10.800 7.560 N Pgap2 n/a
6 TRCN0000262950 TCTTGACAGCCTTCGCCTATT pLKO_005 517 CDS 100% 10.800 7.560 N Pgap2 n/a
7 TRCN0000194557 GCTCTTCGTCATCAACTTCAT pLKO.1 806 CDS 100% 4.950 3.465 N Pgap2 n/a
8 TRCN0000262949 TCCCTATCTCCACGTTCTCTC pLKO_005 1058 3UTR 100% 4.050 2.835 N Pgap2 n/a
9 TRCN0000173445 CGTCATCAACTTCATCTCCTT pLKO.1 812 CDS 100% 2.640 1.848 N Pgap2 n/a
10 TRCN0000181017 GCACATGCCTTTAATCCCAAT pLKO.1 1720 3UTR 100% 4.050 2.025 Y Map6d1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006507641.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11863 pDONR223 100% 87.8% 90.7% None (many diffs) n/a
2 ccsbBroad304_11863 pLX_304 0% 87.8% 90.7% V5 (many diffs) n/a
3 TRCN0000479879 CTTACCATCGGGAACAGGAACCTC pLX_317 46.6% 87.8% 90.7% V5 (many diffs) n/a
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