Construct: ORF TRCN0000479879

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002474.1_s317c1
Derived from:
ccsbBroadEn_11863
DNA Barcode:
CTTACCATCGGGAACAGGAACCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PGAP2 (27315)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479879
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27315 PGAP2 post-GPI attachment to prot... NM_001256239.2 100% 100%
2 human 27315 PGAP2 post-GPI attachment to prot... NM_001346400.2 100% 100%
3 human 27315 PGAP2 post-GPI attachment to prot... XM_006718191.3 100% 100%
4 human 27315 PGAP2 post-GPI attachment to prot... XM_011520002.1 100% 100%
5 human 27315 PGAP2 post-GPI attachment to prot... NM_001256240.2 98.4% 98.4% 514_525del
6 human 27315 PGAP2 post-GPI attachment to prot... NM_001346398.2 98.4% 98.4% 514_525del
7 human 27315 PGAP2 post-GPI attachment to prot... NM_001346405.1 98.4% 98.4% 514_525del
8 human 27315 PGAP2 post-GPI attachment to prot... XM_006718190.3 98.4% 98.4% 514_525del
9 human 27315 PGAP2 post-GPI attachment to prot... XM_024448443.1 86.1% 66.1% 0_1ins31;134_218del
10 human 27315 PGAP2 post-GPI attachment to prot... XM_024448444.1 86.1% 66.1% 0_1ins31;134_218del
11 human 27315 PGAP2 post-GPI attachment to prot... NM_001346402.2 85% 60.9% 1_35del;200_284del;634_645del
12 human 27315 PGAP2 post-GPI attachment to prot... NM_001256235.1 84.8% 65.2% 0_1ins31;134_218del;568_579del
13 human 27315 PGAP2 post-GPI attachment to prot... XM_006718186.2 84.8% 65.2% 0_1ins31;134_218del;568_579del
14 human 27315 PGAP2 post-GPI attachment to prot... NM_001346397.2 81.9% 81.9% 1_153del;667_678del
15 human 27315 PGAP2 post-GPI attachment to prot... NM_001145438.2 81.4% 81.4% 1_171del
16 human 27315 PGAP2 post-GPI attachment to prot... NM_001283038.1 80.3% 80.3% 1_171del;685_696del
17 human 27315 PGAP2 post-GPI attachment to prot... XM_006718185.2 79.7% 63.4% (many diffs)
18 human 27315 PGAP2 post-GPI attachment to prot... NM_014489.4 79.3% 79.3% 158_340del;697_708del
19 human 27315 PGAP2 post-GPI attachment to prot... XM_011520003.2 79.3% 79.3% 158_340del;697_708del
20 human 27315 PGAP2 post-GPI attachment to prot... XM_011520004.2 79.3% 79.3% 158_340del;697_708del
21 human 27315 PGAP2 post-GPI attachment to prot... XM_017017558.1 75% 66.3% (many diffs)
22 human 27315 PGAP2 post-GPI attachment to prot... XM_006718181.3 73.5% 70.8% (many diffs)
23 human 27315 PGAP2 post-GPI attachment to prot... NM_001256238.1 73.1% 67.6% 514_598del;696_697ins139
24 human 27315 PGAP2 post-GPI attachment to prot... XM_024448445.1 73.1% 67.6% 514_598del;696_697ins139
25 human 27315 PGAP2 post-GPI attachment to prot... NM_001346404.1 72.6% 67.1% 514_604del;702_703ins139
26 human 27315 PGAP2 post-GPI attachment to prot... XM_011519996.1 70.4% 51.4% 1_35del;200_467del;817_828del
27 human 27315 PGAP2 post-GPI attachment to prot... XM_011519992.1 67.3% 49.3% 1_35del;200_515del;865_876del
28 human 27315 PGAP2 post-GPI attachment to prot... NM_001256236.1 67.2% 67.2% 1_171del;329_511del;868_879del
29 human 27315 PGAP2 post-GPI attachment to prot... XM_017017560.1 66.9% 5.6% 1_35del;200_327del;774_775ins139
30 human 27315 PGAP2 post-GPI attachment to prot... XM_011519997.1 66.6% 52.2% 0_1ins31;134_449del;799_810del
31 human 27315 PGAP2 post-GPI attachment to prot... XM_011519998.2 66.6% 52.2% 0_1ins31;134_449del;799_810del
32 human 27315 PGAP2 post-GPI attachment to prot... XM_011519999.1 66.6% 52.2% 0_1ins31;134_449del;799_810del
33 human 27315 PGAP2 post-GPI attachment to prot... XM_017017559.1 66% 5.9% (many diffs)
34 human 27315 PGAP2 post-GPI attachment to prot... NM_001346401.2 65.8% 6.4% (many diffs)
35 human 27315 PGAP2 post-GPI attachment to prot... XM_024448446.1 65.8% 6.4% (many diffs)
36 human 27315 PGAP2 post-GPI attachment to prot... NM_001346399.2 64.8% 6.7% (many diffs)
37 human 27315 PGAP2 post-GPI attachment to prot... XM_006718193.2 64.8% 6.7% (many diffs)
38 human 27315 PGAP2 post-GPI attachment to prot... XM_011520006.2 64.8% 6.7% (many diffs)
39 human 27315 PGAP2 post-GPI attachment to prot... XM_011520007.2 64.8% 6.7% (many diffs)
40 human 27315 PGAP2 post-GPI attachment to prot... XM_011519991.2 64.5% 64.5% 1_228del;386_568del
41 human 27315 PGAP2 post-GPI attachment to prot... XM_011519990.2 63.9% 63.9% 1_228del;386_568del;925_936del
42 human 27315 PGAP2 post-GPI attachment to prot... NM_001283039.1 60.7% 6.1% (many diffs)
43 human 27315 PGAP2 post-GPI attachment to prot... NM_001256237.1 60.3% 55.3% 1_171del;685_775del;873_874ins139
44 human 27315 PGAP2 post-GPI attachment to prot... NM_001346403.1 59.6% 54.7% 158_340del;697_787del;885_886ins139
45 human 27315 PGAP2 post-GPI attachment to prot... XM_024448442.1 54.6% 46.6% (many diffs)
46 human 27315 PGAP2 post-GPI attachment to prot... XM_011519994.2 49% 44.6% (many diffs)
47 human 27315 PGAP2 post-GPI attachment to prot... XM_011519993.2 48.8% 44.4% (many diffs)
48 human 27315 PGAP2 post-GPI attachment to prot... NR_104271.2 40.1% 1_117del;282_366del;953_1868del
49 human 27315 PGAP2 post-GPI attachment to prot... NR_045929.2 39.2% (many diffs)
50 human 27315 PGAP2 post-GPI attachment to prot... NR_104272.2 38.9% (many diffs)
51 human 27315 PGAP2 post-GPI attachment to prot... NR_027016.3 38.6% (many diffs)
52 human 27315 PGAP2 post-GPI attachment to prot... NM_001283040.1 38.4% 1.4% (many diffs)
53 human 27315 PGAP2 post-GPI attachment to prot... NR_027018.2 37.5% (many diffs)
54 human 27315 PGAP2 post-GPI attachment to prot... NR_104270.2 37.3% (many diffs)
55 human 27315 PGAP2 post-GPI attachment to prot... NR_045923.2 37.1% (many diffs)
56 human 27315 PGAP2 post-GPI attachment to prot... NR_144427.2 35.6% (many diffs)
57 human 27315 PGAP2 post-GPI attachment to prot... NR_045927.2 35.4% (many diffs)
58 human 27315 PGAP2 post-GPI attachment to prot... NR_144428.2 35% (many diffs)
59 human 27315 PGAP2 post-GPI attachment to prot... NR_027017.4 33.7% (many diffs)
60 human 27315 PGAP2 post-GPI attachment to prot... NR_144429.2 33% (many diffs)
61 human 27315 PGAP2 post-GPI attachment to prot... NR_045925.2 27.8% 1_117del;282_283ins253;615_1530del
62 human 27315 PGAP2 post-GPI attachment to prot... NR_045926.2 27.6% (many diffs)
63 human 27315 PGAP2 post-GPI attachment to prot... NR_144430.2 23.8% (many diffs)
64 mouse 233575 Pgap2 post-GPI attachment to prot... NM_001291358.1 91.3% 94.4% (many diffs)
65 mouse 233575 Pgap2 post-GPI attachment to prot... NM_145583.3 91.3% 94.4% (many diffs)
66 mouse 233575 Pgap2 post-GPI attachment to prot... XM_006507643.3 89.8% 92.9% (many diffs)
67 mouse 233575 Pgap2 post-GPI attachment to prot... XM_006507644.3 89.8% 92.9% (many diffs)
68 mouse 233575 Pgap2 post-GPI attachment to prot... XM_006507645.2 89.8% 92.9% (many diffs)
69 mouse 233575 Pgap2 post-GPI attachment to prot... XM_006507650.3 89.8% 92.9% (many diffs)
70 mouse 233575 Pgap2 post-GPI attachment to prot... XM_006507651.3 89.8% 92.9% (many diffs)
71 mouse 233575 Pgap2 post-GPI attachment to prot... XM_006507642.1 89.1% 92.1% (many diffs)
72 mouse 233575 Pgap2 post-GPI attachment to prot... XM_006507641.3 87.8% 90.7% (many diffs)
73 mouse 233575 Pgap2 post-GPI attachment to prot... XM_017322182.1 87.8% 90.7% (many diffs)
74 mouse 233575 Pgap2 post-GPI attachment to prot... XM_006507646.2 84.3% 83.8% (many diffs)
75 mouse 233575 Pgap2 post-GPI attachment to prot... XM_006507649.3 76.7% 71.9% (many diffs)
76 mouse 233575 Pgap2 post-GPI attachment to prot... XM_006507648.3 75.5% 70.8% (many diffs)
77 mouse 233575 Pgap2 post-GPI attachment to prot... NR_111937.1 33.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
816
ORF length:
750
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgta ccaggtccca ctaccactgg atcgggatgg gaccctggta cggctccgct
121tcaccatggt ggccctggtc acggtctgct gtccacttgt cgccttcctc ttctgcatcc
181tctggtccct gctcttccac ttcaaggaga caacggccac acactgtggg gtgcccaatt
241acctgccctc ggtgagctca gccatcggcg gggaggtgcc ccagcgctac gtgtggcgtt
301tctgcatcgg cctgcactcg gcgcctcgct tcttggtggc cttcgcctac tggaaccact
361acctcagctg cacctccccg tgttcctgct atcgcccgct ctgccgcctc aacttcggcc
421tcaatgtcgt ggagaacctc gcgttgctag tgctcactta tgtctccTCC TCCGAGGACT
481TCACCATCCA CGAAAATGCT TTCATTGTGT TCATTGCCTC ATCCCTCGGG CACATGCTCC
541TCACCTGCAT TCTCTGGCGG TTGACCAAGA AGCACACAGA TCGCAAGTCC TACAGCTGGA
601AACAGCGGCT CTTCATCATC AACTTCATCT CCTTCTTCTC GGCGCTGGCT GTCTACTTTC
661GGCACAACAT GTATTGTGAG GCTGGAGTGT ACACCATCTT TGCCATCCTG GAGTACACTG
721TTGTCTTAAC CAACATGGCG TTCCACATGA CGGCCTGGTG GGACTTCGGG AACAAGGAGC
781TGCTCATAAC CTCTCAGCCT GAGGAAAAGC GATTCTGCCC AACTTTCTTG TACAAAGTGG
841TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
901CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
961ACTTACCATC GGGAACAGGA ACCTCACGCG TTAAGTCgac aatcaacctc tggattacaa
1021aatttgtgaa agatt

Download FASTA (ORF) (Full)