Transcript: Mouse XM_006508100.2

PREDICTED: Mus musculus potassium channel tetramerisation domain containing 21 (Kctd21), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Kctd21 (622320)
Length:
3093
CDS:
199..981

Additional Resources:

NCBI RefSeq record:
XM_006508100.2
NBCI Gene record:
Kctd21 (622320)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006508100.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000255107 GAGAAGAATGCCATGCTTAAC pLKO_005 517 CDS 100% 10.800 15.120 N Kctd21 n/a
2 TRCN0000255109 CAATAAGATCATTCGGTTAAT pLKO_005 948 CDS 100% 13.200 10.560 N Kctd21 n/a
3 TRCN0000267542 TGGCAAAGTGTTCCGCTATAT pLKO_005 354 CDS 100% 13.200 10.560 N Kctd21 n/a
4 TRCN0000255108 GCCAGGGTAACTGCTTCATTG pLKO_005 326 CDS 100% 10.800 8.640 N Kctd21 n/a
5 TRCN0000255106 AGCAGATCTGTGGTCATATTC pLKO_005 2776 3UTR 100% 13.200 9.240 N Kctd21 n/a
6 TRCN0000139768 CCTTGGCTCTAAGCTCTTCTA pLKO.1 672 CDS 100% 4.950 3.465 N KCTD21 n/a
7 TRCN0000140024 GATCAACAGCTTCCAGGTCTT pLKO.1 846 CDS 100% 4.050 2.835 N KCTD21 n/a
8 TRCN0000139705 CAATGGCAATCTCTCCTCCAT pLKO.1 699 CDS 100% 2.640 1.848 N KCTD21 n/a
9 TRCN0000139215 CTTCTACTGCTCCAATGGCAA pLKO.1 687 CDS 100% 2.640 1.848 N KCTD21 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006508100.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05352 pDONR223 100% 91.4% 99.6% None (many diffs) n/a
2 ccsbBroad304_05352 pLX_304 0% 91.4% 99.6% V5 (many diffs) n/a
3 TRCN0000476990 CGGTTAGCGTGAGTGACCGTCTCT pLX_317 47.3% 91.4% 99.6% V5 (many diffs) n/a
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