Transcript: Mouse XM_006513980.2

PREDICTED: Mus musculus coiled-coil domain containing 53 (Ccdc53), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Washc3 (67282)
Length:
1020
CDS:
138..674

Additional Resources:

NCBI RefSeq record:
XM_006513980.2
NBCI Gene record:
Washc3 (67282)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006513980.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000176446 CAAACGGATCACATTCTGAAA pLKO.1 421 CDS 100% 4.950 6.930 N Washc3 n/a
2 TRCN0000182823 CTGAAACCACTTCGGAGCAAA pLKO.1 436 CDS 100% 4.950 6.930 N Washc3 n/a
3 TRCN0000424333 GATCCACGGTATGCCAGATAC pLKO_005 534 CDS 100% 10.800 7.560 N Washc3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006513980.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08195 pDONR223 100% 80.1% 77.6% None (many diffs) n/a
2 ccsbBroad304_08195 pLX_304 0% 80.1% 77.6% V5 (many diffs) n/a
3 TRCN0000473745 GAATACGAATTTGGGGTATACTTA pLX_317 93.5% 80.1% 77.6% V5 (many diffs) n/a
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