Construct: ORF TRCN0000473745
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012736.1_s317c1
- Derived from:
- ccsbBroadEn_08195
- DNA Barcode:
- GAATACGAATTTGGGGTATACTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WASHC3 (51019)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473745
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51019 | WASHC3 | WASH complex subunit 3 | NM_016053.4 | 99.8% | 99.4% | 329A>G |
| 2 | human | 51019 | WASHC3 | WASH complex subunit 3 | NM_001301107.1 | 99.3% | 98.9% | 321_322insCAG;326A>G |
| 3 | human | 51019 | WASHC3 | WASH complex subunit 3 | XM_011538423.1 | 85.6% | 85.5% | 329A>G;502_505delGTGA;507_508ins79 |
| 4 | human | 51019 | WASHC3 | WASH complex subunit 3 | XM_017019383.1 | 82.8% | 82.4% | 49_50ins99;230A>G |
| 5 | human | 51019 | WASHC3 | WASH complex subunit 3 | XM_024448998.1 | 82.3% | 81.9% | 49_50ins99;222_223insCAG;227A>G |
| 6 | mouse | 67282 | Washc3 | WASH complex subunit 3 | NM_026070.3 | 87.6% | 89.6% | (many diffs) |
| 7 | mouse | 67282 | Washc3 | WASH complex subunit 3 | NM_001122960.1 | 87.2% | 89.1% | (many diffs) |
| 8 | mouse | 67282 | Washc3 | WASH complex subunit 3 | XM_006513980.2 | 80.1% | 77.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 648
- ORF length:
- 582
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgaggacggg cttcctctca tggggTCAGG CATAGACCTG ACCAAGGTGC 121 CAGCTATTCA ACAGAAAAGA ACGGTGGCTT TTCTAAACCA ATTTGTGGTG CACACTGTAC 181 AGTTCCTCAA CCGCTTTTCT ACAGTTTGTG AGGAGAAACT GGCAGACCTT TCACTTCGTA 241 TCCAACAAAT TGAAACAACT CTCAATATTT TAGATGCAAA GTTGTCATCT ATCCCAGGCC 301 TAGATGATGT CACAGTTGAA GTATCTCCTT TAAATGTCAC CAGTGTCACA AATGGAGCAC 361 ATCCTGAAGC CACTTCAGAG CAACCACAGC AGAGCAGTAC ACAAGACTCT GGACTACAGG 421 AAAGTGAAGT ATCAGCAGAA AATATCTTAA CTGTAGCCAA GGATCCAAGA TATGCCAGAT 481 ATCTCAAAAT GGTTCAAGTG GGTGTACCAG TGATGGCAAT AAGAAACAAA ATGATATCAG 541 AAGGACTAGA CCCAGATCTT CTTGAGAGGC CAGATGCTCC AGTGCCTGAT GGCGAAAGTG 601 AGAAAACTGT AGAAGAAAGT TCAGATAGCG AATCTTCTTT TAGTGATTAC CCAACTTTCT 661 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 721 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 781 GTGGAAAGGA CGAGAATACG AATTTGGGGT ATACTTAACG CGTTAAGTCg acaatcaacc 841 tctggattac aaaatttgtg aaagatt