Transcript: Mouse XM_006516662.3

PREDICTED: Mus musculus 5-methyltetrahydrofolate-homocysteine methyltransferase (Mtr), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mtr (238505)
Length:
3394
CDS:
338..3313

Additional Resources:

NCBI RefSeq record:
XM_006516662.3
NBCI Gene record:
Mtr (238505)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006516662.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000251689 AGCTCTGGGTCCGACTAATAA pLKO_005 730 CDS 100% 15.000 12.000 N Mtr n/a
2 TRCN0000251687 GTATAACTCAGCCTGATATTA pLKO_005 510 CDS 100% 15.000 12.000 N Mtr n/a
3 TRCN0000251688 CCAGTGAAGCCCACGTTTATT pLKO_005 3149 CDS 100% 15.000 10.500 N Mtr n/a
4 TRCN0000424139 GTTTCTACCTCAGGTTATAAA pLKO_005 2485 CDS 100% 15.000 10.500 N MTR n/a
5 TRCN0000429125 CAGTGAAGCCCACGTTTATTG pLKO_005 3150 CDS 100% 13.200 9.240 N MTR n/a
6 TRCN0000251686 TGGACCACAAAGCAGATATAA pLKO_005 2760 CDS 100% 15.000 9.000 N Mtr n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006516662.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13904 pDONR223 100% 69.3% 44% None (many diffs) n/a
2 ccsbBroad304_13904 pLX_304 0% 69.3% 44% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000481034 TCTCGTACTTCTAGTCTTGCAGTC pLX_317 11.2% 69.3% 44% V5 (not translated due to prior stop codon) (many diffs) n/a
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